ArticleJournal of bacteriology2022
Mutational Analysis of
Article in Journal of bacteriology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 18 citations in OpenAlex.
- The calcium signal regulatory network and the roles of calcium-binding proteins in bacteria.World journal of microbiology & biotechnology · 2026Review
- Genetic and biochemical analyses reveal direct interactions between LitR and genes important forJournal of bacteriology · 2025Article
- Variation in accessory and horizontal gene transfer-associated genes drives lucinid endosymbiont diversity.FEMS microbiology ecology · 2025Article
- Calcium signaling controls early stage biofilm formation and dispersal inJournal of bacteriology · 2025Article
- Host-Associated Biofilms:Microorganisms · 2025Review
- Generation and validation of a versatile inducible multiplex CRISPRi system to examine bacterial regulation in the Euprymna-Vibrio fischeri symbiosis.Archives of microbiology · 2025Article
- Interplay of virulence factors and signaling molecules: albumin and calcium-mediated biofilm regulation inJournal of bacteriology · 2025Article
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- Cyclic Diguanylate in the Wild: Roles During Plant and Animal Colonization.Annual review of microbiology · 2024Review
- Functional analysis of cyclic diguanylate-modulating proteins inbioRxiv : the preprint server for biology · 2024Article
- Environmental magnesium ion affects global gene expression, motility, biofilm formation and virulence ofBiofilm · 2024Article
- A Label-Free Approach for Relative Spatial Quantitation of c-di-GMP in Microbial Biofilms.Analytical chemistry · 2024Article
- Lighting the way: how theJournal of bacteriology · 2024Review
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- A label-free approach for relative spatial quantitation of c-di-GMP in microbial biofilms.bioRxiv : the preprint server for biology · 2023Article
- Bioinformatic and functional characterization of cyclic-di-GMP metabolic proteins inFrontiers in microbiology · 2023Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The symbiont Vibrio fischeri uses motility to colonize its host. In numerous bacterial species, motility is negatively controlled by cyclic-di-GMP (c-di-GMP), which is produced by diguanylate cyclases (DGCs) with GGDEF domains and degraded by phosphodiesterases with either EAL or HD-GYP domains. To begin to decode the functions of the 50 Vibrio fischeri genes with GGDEF, EAL, and/or HD-GYP domains, we deleted each gene and assessed each mutant's migration through tryptone broth salt (TBS) soft agar medium containing or lacking magnesium (Mg) and calcium (Ca), which are known to influence V. fischeri motility. We identified 6, 13, and 16 mutants with altered migration in TBS-Mg, TBS, and TBS-Ca soft agar, respectively, a result that underscores the importance of medium conditions in assessing gene function. A biosensor-based assay revealed that Mg and Ca affected c-di-GMP levels negatively and positively, respectively; the severe decrease in c-di-GMP caused by Mg addition correlates with its strong positive impact on bacterial migration. A mutant defective for
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.