Evidence map›Paper›PMID 35758269›Full record

ArticleBioengineered2022

Preparation of a nanopearl powder/C-HA (chitosan-hyaluronic acid)/rhBMP-2 (recombinant human bone morphogenetic protein-2) composite artificial bone material and a preliminary study of its effects on MC3T3-E1 cells.

Wenbo Zhang, Pu Xu, Yanan Cheng, Yanlan Yang, Qiuhua Mao, Zuogeng Chen

RetractedAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineered, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Wenbo ZhangDepartment of Periodontitis, Affiliated Haikou Hospital, Xiangya Medical School, Central South University • Hainan Provincial Stomatology Centre, Haikou, Hainan, China.
Pu XuDepartment of Oral Implantation, Affiliated Haikou Hospital, Xiangya Medical School, Central South University Hainan Provincial Stomatology Centre, Haikou, Hainan, China.
Yanan ChengDepartment of Oral Implantation, Affiliated Haikou Hospital, Xiangya Medical School, Central South University Hainan Provincial Stomatology Centre, Haikou, Hainan, China.
Yanlan YangDepartment of Oral Implantation, Affiliated Haikou Hospital, Xiangya Medical School, Central South University Hainan Provincial Stomatology Centre, Haikou, Hainan, China.
Qiuhua MaoDepartment of Periodontitis, Affiliated Haikou Hospital, Xiangya Medical School, Central South University • Hainan Provincial Stomatology Centre, Haikou, Hainan, China.
Zuogeng ChenDepartment of Oral Implantation, Affiliated Haikou Hospital, Xiangya Medical School, Central South University Hainan Provincial Stomatology Centre, Haikou, Hainan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A nanopearl powder/C-HA (chitosan-hyaluronic acid)/rhBMP-2 (recombinant human bone morphogenetic protein-2) composite artificial bone material was prepared, and its biological properties were evaluated. The nanopearl powder/C-HA/rhBMP-2 composite porous artificial bone material was prepared using the freeze-drying method after the nanopearl powder was prepared using mechanical ball milling. The particle was measured with a transmission electron microscope, its surface morphology and pore size were observed under a scanning electron microscope. The porosity of the artificial bone was determined using pycnometry, a compression performance test was conducted with a universal testing machine, and XRD (X-ray diffraction) patterns were recorded to examine the crystal form of the pearl powder in the composite artificial bone. Finally, the artificial bone was cocultured with mouse MC3T3-E1 cells to investigate its effects on cell proliferation and differentiation and the expression of osteogenesis-related genes. The pearl powder prepared in this experiment had a particle size in the nanometer range. This nanopearl powder, along with C-HA and rhBMP-2, was compounded into the nanopearl powder/C-HA/rhBMP-2 composite artificial bone, showing pore sizes of 188.53 ± 15.32 μm, a porosity of 86.43 ± 2.78% and a compressive strength of 0.342 ± 0.024 MPa. Notably, rhBMP-2 was released from the artificial bone in a sustained manner. Moreover, this artificial bone promoted the adhesion, proliferation, and differentiation of MC3T3-E1 cells and upregulated the expression of ColαI (collagen α1), OCN (osteocalcin), OPN (osteopontin) and Runx2 (runt-related gene 2). Conclusively, this nanopearl powder/C-HA/rhBMP-2 composite artificial bone material showed good performance and cytocompatibility, suggesting that it can be used for bone tissue engineering.

Indexed as

ChitosanAnimalsBone Morphogenetic Protein 2Calcium CarbonateHumansHyaluronic AcidMicePorosityPowdersRecombinant ProteinsTissue ScaffoldsTransforming Growth Factor betaBone Morphogenetic Protein 2Calcium CarbonateChitosanHyaluronic Acidpearl powderPowdersrecombinant human bone morphogenetic protein-2Recombinant ProteinsTransforming Growth Factor betachitosancomposite artificial bonehyaluronic acidNanopearl powderrecombinant human bone morphogenetic protein

Identifiers

PMID35758269
PMCPMC9342380

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.