Evidence map›Paper›PMID 35758052›Full record

ArticleVirulence2022

Hearing loss in outbred Hartley guinea pigs experimentally infected with Pichinde virus as a surrogate model of human mammarenaviral hemorrhagic fevers.

Morgan Brisse, Claudia Fernández-Alarcón, Qinfeng Huang, Natalie Kirk, Mark R Schleiss, Yuying Liang, Hinh Ly

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virulence, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Morgan BrisseBiochemistry, Molecular Biology and Biophysics Graduate Program, University of Minnesota, Twin Cities, Minnesota, USA.
Claudia Fernández-AlarcónDepartment of Pediatrics, School of Medicine University of Minnesota, Twin Cities, Minnesota, USA.
Qinfeng HuangDepartment of Veterinary and Biomedical Sciences College of Veterinary Medicine, University of Minnesota, Twin Cities, Minnesota, USA.
Natalie KirkComparative and Molecular Biosciences Graduate Program, University of Minnesota, Twin Cities, Minnesota, USA.
Mark R SchleissDepartment of Pediatrics, School of Medicine University of Minnesota, Twin Cities, Minnesota, USA.
Yuying LiangDepartment of Veterinary and Biomedical Sciences College of Veterinary Medicine, University of Minnesota, Twin Cities, Minnesota, USA.
Hinh LyBiochemistry, Molecular Biology and Biophysics Graduate Program, University of Minnesota, Twin Cities, Minnesota, USA.
University of Minnesota · USTwin Cities Orthopedics · US

Funding

Training in PharmacoNeuroImmune Substance Abuse ResearchT32DA007097 · NIDA · UNIVERSITY OF MINNESOTA TWIN CITIES · PI MOLITOR, THOMAS WILLIAM · 1985 to 2021
$13.1M
Comparative Medicine and Pathology TrainingT32OD010993 · OD · UNIVERSITY OF MINNESOTA · PI DAVID R BROWN, Cathy S. Carlson · 2012 to 2026
$6.2M
Mechanism of Lassa fever virus Z protein in immune suppression and viral virulenceR01AI131586 · NIAID · UNIVERSITY OF MINNESOTA · PI LIANG, YUYING, LY, HINH · 2017 to 2021
$3.8M
NIAID NIH HHS R01 AI131586NIDA NIH HHS T32 DA007097NIH HHS T32 OD010993
6 · The paper itself

Abstract

Lassa fever (LF) is a neglected tropical disease that is caused by Lassa virus (LASV), a human hemorrhagic fever-causing mammarenavirus. A notable sequela of LF is sensorineural hearing loss (SNHL) that can develop in about 33% of the patients. Animal models of LF-associated SNHL have been limited in size and scope because LASV is a biosafety level 4 (BSL4) pathogen that requires its handling in a high biocontainment laboratory. In this report, we describe the development of an alternative arenavirus hearing loss model by infecting outbred Hartley guinea pigs with a virulent strain (rP18) of the Pichinde virus (PICV), which is a guinea pig-adapted mammarenavirus that has been used as a surrogate model of mammarenaviral hemorrhagic fevers in a conventional (BSL2) laboratory. By measuring auditory brainstem response (ABR) throughout the course of the virulent rP18 PICV infection, we noticed that some of the animals experienced an acute but transient level of hearing loss. Cochleae of hearing-impaired animals, but not of controls, had demonstrable viral RNA by quantitative RT-PCR, indicating the presence of virus in the affected inner ear with no overt histopathological changes. In contrast, neither the outbred Hartley guinea pigs infected with a known avirulent strain (rP2) of PICV nor those that were mock-infected showed any evidence of hearing loss or viral infection of the inner ear. This is the first report of an immunocompetent small animal model of mammarenavirus-induced hearing loss that can be used to evaluate potential therapeutics against virus-induced hearing impairment under a conventional laboratory setting.

Indexed as

Hearing LossLassa FeverPichinde virusAnimalsDisease Models, AnimalFeverGuinea PigsHumansLassa virusArenavirusguinea pigsLassa virusMammarenavirusPichinde virusSensorineural hearing loss (SNHL)Virus-induced hearing loss

Identifiers

PMID35758052
PMCPMC9794012
OpenAlexW4283585740

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.