Evidence map›Paper›PMID 35757844›Full record

ArticleClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2022

A method based on plateletpheresis to obtain functional platelet, CD3

Carmela Pablo-Torres, María Isabel Delgado-Dolset, Javier Sanchez-Solares, Leticia Mera-Berriatua, Lucía Núñez Martín Buitrago, Mar Reaño Martos, José Luis Bueno, Maria M Escribese, Domingo Barber, Cristina Gomez-Casado

Open access · bronzeAbstract read
In one paragraph

Article in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

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  6. A method based on plateletpheresis to obtain functional platelet, CD3Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Carmela Pablo-TorresInstitute of Applied Molecular Medicine (IMMA) Nemesio Díez, Department of Basic Medical Sciences, School of Medicine, San Pablo-CEU University, CEU Universities, Boadilla del Monte, Spain.ORCID 0000-0003-2861-5763
María Isabel Delgado-DolsetInstitute of Applied Molecular Medicine (IMMA) Nemesio Díez, Department of Basic Medical Sciences, School of Medicine, San Pablo-CEU University, CEU Universities, Boadilla del Monte, Spain.ORCID 0000-0001-7924-5454
Javier Sanchez-SolaresInstitute of Applied Molecular Medicine (IMMA) Nemesio Díez, Department of Basic Medical Sciences, School of Medicine, San Pablo-CEU University, CEU Universities, Boadilla del Monte, Spain.
Leticia Mera-BerriatuaInstitute of Applied Molecular Medicine (IMMA) Nemesio Díez, Department of Basic Medical Sciences, School of Medicine, San Pablo-CEU University, CEU Universities, Boadilla del Monte, Spain.
Lucía Núñez Martín BuitragoDepartment of Hematology and Hemotherapy, Puerta de Hierro-Majadahonda University Hospital, Madrid, Spain.
Mar Reaño MartosDepartment of Allergy and Immunology, Puerta de Hierro-Majadahonda University Hospital, Madrid, Spain.
José Luis BuenoDepartment of Hematology and Hemotherapy, Puerta de Hierro-Majadahonda University Hospital, Madrid, Spain.
Maria M EscribeseInstitute of Applied Molecular Medicine (IMMA) Nemesio Díez, Department of Basic Medical Sciences, School of Medicine, San Pablo-CEU University, CEU Universities, Boadilla del Monte, Spain.ORCID 0000-0001-5057-5150
Domingo BarberInstitute of Applied Molecular Medicine (IMMA) Nemesio Díez, Department of Basic Medical Sciences, School of Medicine, San Pablo-CEU University, CEU Universities, Boadilla del Monte, Spain.ORCID 0000-0002-5488-5700
Cristina Gomez-CasadoInstitute of Applied Molecular Medicine (IMMA) Nemesio Díez, Department of Basic Medical Sciences, School of Medicine, San Pablo-CEU University, CEU Universities, Boadilla del Monte, Spain.ORCID 0000-0002-7707-6367
Universidad San Pablo CEU · ESHospital Universitario Puerta de Hierro Majadahonda · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn previous studies with peripheral blood cells, platelet factors were found to be associated with severe allergic phenotypes. A reliable method yielding highly concentrated and pure platelet samples is usually not available for immunological studies. Plateletpheresis is widely used in the clinics for donation purposes. In this study, we designed a protocol based on plateletpheresis to obtain Platelet-Rich Plasma (PRP), Platelet-Poor Plasma (PPP) as well as CD3

methodsTwenty-seven subjects were voluntarily subjected to plateletpheresis. PRP, PPP and blood cell concentrate contained in a leukocyte reduction system chamber (LRSC) were obtained in this process. CD3

resultsA reliable high yield method to obtain matched samples of PRP, PPP, CD3

conclusionsWe have developed a method that yields not only high content and pure platelet samples from a single donor but also CD3

Indexed as

Blood PlateletsPlateletpheresisLeukocytesRNARNAleukocyte reduction system chamber (LRSC)multiplexplateletpheresisplatelet-rich plasma (PRP)plateletstranscriptomics

Identifiers

PMID35757844
PMCPMC9796013
OpenAlexW4283585945

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.