Evidence map›Paper›PMID 35757178›Full record

ArticleComprehensive psychoneuroendocrinology2022

Methodological considerations for ghrelin isoforms assay in clinical evaluation in anorexia nervosa.

C Tezenas du Montcel, P Duriez, N Lebrun, D Grouselle, B de Grimaudet, R Dardennes, J Epelbaum, M Cuenca, O Viltart, P Gorwood and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Comprehensive psychoneuroendocrinology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
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  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

C Tezenas du MontcelUniversité Paris Cité, UMR-S 1266 INSERM, Institut de Psychiatrie et Neuroscience de Paris (IPNP), F-75014, Paris, France.
P DuriezUniversité Paris Cité, UMR-S 1266 INSERM, Institut de Psychiatrie et Neuroscience de Paris (IPNP), F-75014, Paris, France.
N LebrunUniversité Paris Cité, UMR-S 1266 INSERM, Institut de Psychiatrie et Neuroscience de Paris (IPNP), F-75014, Paris, France.
D GrouselleUniversité Paris Cité, UMR-S 1266 INSERM, Institut de Psychiatrie et Neuroscience de Paris (IPNP), F-75014, Paris, France.
B de GrimaudetBertin Technologies, F-78180, Montigny-le-Bretonneux, France.
R DardennesClinique des Maladies Mentales et de L'Encéphale, GHU Paris Psychiatrie et Neurosciences, Hôpital Sainte-Anne, F-75014, Paris, France.
J EpelbaumUniversité Paris Cité, UMR-S 1266 INSERM, Institut de Psychiatrie et Neuroscience de Paris (IPNP), F-75014, Paris, France.
M CuencaCentre de Recherche Clinique, GHU Paris Psychiatrie et Neurosciences, Hôpital Sainte-Anne, F-75014, Paris, France.
O ViltartUniversité Paris Cité, UMR-S 1266 INSERM, Institut de Psychiatrie et Neuroscience de Paris (IPNP), F-75014, Paris, France.
P GorwoodUniversité Paris Cité, UMR-S 1266 INSERM, Institut de Psychiatrie et Neuroscience de Paris (IPNP), F-75014, Paris, France.
V TolleUniversité Paris Cité, UMR-S 1266 INSERM, Institut de Psychiatrie et Neuroscience de Paris (IPNP), F-75014, Paris, France.
Inserm · FRCentre National de la Recherche Scientifique · FRCentre Hospitalier Sainte-Anne · FRUniversité Paris Cité · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The growing interest concerning the role of metabolic sensors in various eating disorders requires the implementation of a strict methodology to collect, store and process blood samples in clinical studies. In particular, measurement of isoforms of the appetite-stimulating hormone, ghrelin, has been challenging in clinical settings. Indeed the acyl ghrelin (AG) isoform is rapidly degraded into desacyl ghrelin (DAG) by blood esterases, thus optimal conditions for the conservation of AG and accurate determination of AG/DAG ratio should be used. Here, we compared different protease inhibitors (Aprotinin, PHMB, AEBSF) during blood collection, increasing delays (0-180 min) before centrifugation, plasma supplementation with various HCl concentrations, storage durations of frozen plasma (8 and 447 days) and immunoenzyme-assay procedures (one-step

Indexed as

Anorexia nervosaAppetiteGhrelinImmunoassayPulsatile secretion

Identifiers

PMID35757178
PMCPMC9227986
OpenAlexW4280555836

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.