Evidence map›Paper›PMID 35756525›Full record

ArticleChemical science2022

Mapping paratopes of nanobodies using native mass spectrometry and ultraviolet photodissociation.

Luis A Macias, Xun Wang, Bryan W Davies, Jennifer S Brodbelt

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 10 citations in OpenAlex.

  1. Analytical applications of gas-phase ion chemistry enabled by mass spectrometry.Analytical methods : advancing methods and applications · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Luis A MaciasDepartment of Chemistry, University of Texas at Austin Austin TX 78712 USA jbrodbelt@cm.utexas.edu.ORCID https://orcid.org/0000-0003-1949-6029
Xun WangDepartment of Molecular Biosciences, University of Texas at Austin Austin TX 78712 USA.
Bryan W DaviesDepartment of Molecular Biosciences, University of Texas at Austin Austin TX 78712 USA.
Jennifer S BrodbeltDepartment of Chemistry, University of Texas at Austin Austin TX 78712 USA jbrodbelt@cm.utexas.edu.ORCID https://orcid.org/0000-0003-3207-0217
The University of Texas at Austin · US

Funding

Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological MoleculesR35GM139658 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI BRODBELT, JENNIFER S. · 2021 to 2025
$2.9M
Investigating nanobodies to target multidrug resistant bacterial pathogensR01AI148419 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI DAVIES, BRYAN WILLIAM · 2020 to 2023
$2.8M
A High-Throughput Molecular Platform for Antimicrobial Discovery and StudyR01AI125337 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI DAVIES, BRYAN WILLIAM · 2016 to 2020
$2.0M
Investigation of capsule interactions that promote antimicrobial peptide activityR21AI159203 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI DAVIES, BRYAN WILLIAM · 2021 to 2022
$429k
Implementing Photodissociation Mass Spectrometry to study Disease and Lipid StructureF31CA257404 · NCI · UNIVERSITY OF TEXAS AT AUSTIN · PI MACIAS, LUIS A · 2021 to 2022
$49k
NCI NIH HHS F31 CA257404NIAID NIH HHS R01 AI125337NIAID NIH HHS R01 AI148419NIAID NIH HHS R21 AI159203NIGMS NIH HHS R35 GM139658
6 · The paper itself

Abstract

Following immense growth and maturity of the field in the past decade, native mass spectrometry has garnered widespread adoption for the structural characterization of macromolecular complexes. Routine analysis of biotherapeutics by this technique has become commonplace to assist in the development and quality control of immunoglobulin antibodies. Concurrently, 193 nm ultraviolet photodissociation (UVPD) has been developed as a structurally sensitive ion activation technique capable of interrogating protein conformational changes. Here, UVPD was applied to probe the paratopes of nanobodies, a class of single-domain antibodies with an expansive set of applications spanning affinity reagents, molecular imaging, and biotherapeutics. Comparing UVPD sequence fragments for the free nanobodies

Identifiers

PMID35756525
PMCPMC9172568
OpenAlexW4283662458

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.