Evidence map›Paper›PMID 35756123›Full record

ArticleHeliyon2022

Neutrophil extracellular traps (NETs) reduce the diffusion of doxorubicin which may attenuate its ability to induce apoptosis of ovarian cancer cells.

Kohei Tamura, Hideyo Miyato, Rihito Kanamaru, Ai Sadatomo, Kazuya Takahashi, Hideyuki Ohzawa, Takahiro Koyanagi, Yasushi Saga, Yuji Takei, Hiroyuki Fujiwara and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Neutrophil extracellular traps (NETosis) in gynecologic cancers: from pathogenesis to therapeutic opportunities: a narrative review.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026
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  8. Harnessing myeloid cells in cancer.Molecular cancer · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Kohei TamuraDepartment of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Japan.
Hideyo MiyatoDepartment of Surgery, Jichi Medical University, Shimotsuke, Japan.
Rihito KanamaruDepartment of Surgery, Jichi Medical University, Shimotsuke, Japan.
Ai SadatomoDepartment of Surgery, Jichi Medical University, Shimotsuke, Japan.
Kazuya TakahashiDepartment of Surgery, Jichi Medical University, Shimotsuke, Japan.
Hideyuki OhzawaDepartment of Clinical Oncology, Jichi Medical University, Shimotsuke, Japan.
Takahiro KoyanagiDepartment of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Japan.
Yasushi SagaDepartment of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Japan.
Yuji TakeiDepartment of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Japan.
Hiroyuki FujiwaraDepartment of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Japan.
Alan Kawarai LeforDepartment of Surgery, Jichi Medical University, Shimotsuke, Japan.
Naohiro SataDepartment of Surgery, Jichi Medical University, Shimotsuke, Japan.
Joji KitayamaDepartment of Surgery, Jichi Medical University, Shimotsuke, Japan.
Jichi Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: Although neutrophil extracellular traps (NETs) are present in various tumors, their roles in tumor biology have not been clarified yet. In this study, we examined how NETs affect the pharmacokinetics and effects of doxorubicin (DOX). Methods: NETs were generated by neutrophils stimulated with phorbol 12-myristate 13-acetate (PMA) or lipopolysaccharide (LPS). DOX was added to NETs and their distribution was observed under fluorescein microscopy, and the diffusion of DOX through 3 μM pores from lower to upper chambers was evaluated with a fluorescence-based assay. Ovarian cancer cells, KOC-2S and SKOV3, were embedded in collagen gel droplets and cultured in 3D way and their apoptosis was examined with flow cytometry. Results: DOX was mostly co-localized with NETs. The transfer of DOX to upper chambers increased over time, which was significantly decreased by the presence of neutrophils stimulated with PMA or LPS in the lower chamber. DOX outside of the gel increased the rates of annexin V (+) apoptotic cells, which were significantly reduced by the addition of LPS-stimulated neutrophils in media both in KOC-2S and SKOV3. The reduced diffusion and apoptosis were mostly restored by the destruction of the NETs structure with 1000 u/ml DNAse I. Conclusion: NETs efficiently trap and inhibit the diffusion of DOX which may attenuate its ability to induce apoptosis of ovarian cancer cells. Degradation of NETs with DNAse I may augment the response of ovarian cancer to DOX.

Indexed as

ChemosensitivityDoxorubicinNeutrophil extracellular trapsPharmacokinetics

Identifiers

PMID35756123
PMCPMC9218137
OpenAlexW4282947077

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.