Evidence map›Paper›PMID 35755251›Full record

ArticleFrontiers in chemistry2022

Investigations Into Chemically Stabilized Four-Letter DNA for DNA-Encoded Chemistry.

Marco Potowski, Verena B K Kunig, Lukas Eberlein, Mateja Klika Škopić, Alexandros Vakalopoulos, Stefan M Kast, Andreas Brunschweiger

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Marco PotowskiDepartment of Chemistry and Chemical Biology, Medicinal Chemistry, TU Dortmund University, Dortmund, Germany.
Verena B K KunigDepartment of Chemistry and Chemical Biology, Medicinal Chemistry, TU Dortmund University, Dortmund, Germany.
Lukas EberleinDepartment of Chemistry and Chemical Biology, Physical Chemistry, TU Dortmund University, Dortmund, Germany.
Mateja Klika ŠkopićDepartment of Chemistry and Chemical Biology, Medicinal Chemistry, TU Dortmund University, Dortmund, Germany.
Alexandros VakalopoulosBayer AG, Pharmaceuticals, Research and Development, Synthetic Modalities, Wuppertal, Germany.
Stefan M KastDepartment of Chemistry and Chemical Biology, Physical Chemistry, TU Dortmund University, Dortmund, Germany.
Andreas BrunschweigerDepartment of Chemistry and Chemical Biology, Medicinal Chemistry, TU Dortmund University, Dortmund, Germany.
TU Dortmund University · DEBayer (Germany) · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA-encoded libraries are a prime technology for target-based small molecule screening. Native DNA used as genetic compound barcode is chemically vulnerable under many reaction conditions. DNA barcodes that are composed of pyrimidine nucleobases, 7-deazaadenine, and 7-deaza-8-azaguanine have been investigated for their suitability for encoded chemistry both experimentally and computationally. These four-letter barcodes were readily ligated by T4 ligation, amplifiable by Taq polymerase, and the resultant amplicons were correctly sequenced. Chemical stability profiling showed a superior chemical stability compared to native DNA, though higher susceptibility to depurination than a three-letter code based on pyrimidine DNA and 7-deazaadenine.

Indexed as

chemically modified nucleobaseDNA-encoded chemistryDNA-encoded libraryheterocyclic chemistrymulti-component reaction

Identifiers

PMID35755251
PMCPMC9218945
OpenAlexW4281824035

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.