Measuring EGFR plasma membrane density, stability, internalization, and recycling in alive adherent cells by cell surface ELISA.

Jalal M Kazan, Ariadna Pollato-Blanco, Gergely L Lukacs, Arnim Pause

Open access · goldAbstract read

In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 94% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Jalal M KazanGoodman Cancer Institute, McGill University, Montreal, QC H3A 1A3, Canada; Biochemistry Department, McGill University, Montreal, QC H3G 1Y6, Canada.

Ariadna Pollato-BlancoGoodman Cancer Institute, McGill University, Montreal, QC H3A 1A3, Canada; Biochemistry Department, McGill University, Montreal, QC H3G 1Y6, Canada.

Gergely L LukacsPhysiology Department, McGill University, Montreal, QC H3G 1Y6, Canada. Electronic address: gergely.lukacs@mcgill.ca.

Arnim PauseGoodman Cancer Institute, McGill University, Montreal, QC H3A 1A3, Canada; Biochemistry Department, McGill University, Montreal, QC H3G 1Y6, Canada. Electronic address: arnim.pause@mcgill.ca.

McGill University · CA

Funding

CIHR 245969

6 · The paper itself

Abstract

EGFR cell surface density, stability, internalization, and recycling can be measured by cell surface ELISA (cs-ELISA). Performing this experiment on ice impedes receptor internalization; thus the physiological cell surface receptor levels can be measured by cs-ELISA. Cell surface EGFR levels are detected by measuring Amplex Red fluorescence intensity. Although cell surface receptor levels can be measured by flow cytometry, cs-ELISA does not include cell dissociation steps that might affect cell surface receptor levels. For complete details on the use and execution of this protocol, please refer to Kazan et al. (2019).

Indexed as

ErbB ReceptorsReceptors, Cell SurfaceCell MembraneEnzyme-Linked Immunosorbent AssayFlow CytometryErbB ReceptorsReceptors, Cell SurfaceCell-based AssaysCell BiologyCell MembraneMolecular/Chemical ProbesSignal Transduction

Identifiers

PMID35755125

DOI10.1016/j.xpro.2022.101475

PMCPMC9213824

OpenAlexW4282970118

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.