Evidence map›Paper›PMID 35755037›Full record

ArticleFrontiers in medicine2022

Rapid and Sensitive Qualitative Duoplex Real-Time PCR Method for Discriminatory and Confirmatory Diagnosis of HTLV-1 and HTLV-2 Infections: Brazilian Multicentric Study.

Mauricio Cristiano Rocha-Junior, Evandra Strazza Rodrigues, Svetoslav Nanev Slavov, Tatiane Assone, Maíra Pedreschi, Debora Glenda Lima de La Roque, Maisa Sousa, Viviana Olavarria, Bernardo Galvão-Castro, Benedito Antonio Lopes da Fonseca and 6 more

Abstract read
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In one paragraph

Article in Frontiers in medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Mauricio Cristiano Rocha-JuniorRegional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.
Evandra Strazza RodriguesRegional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.
Svetoslav Nanev SlavovRegional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.
Tatiane AssoneLaboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.
Maíra PedreschiLaboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.
Debora Glenda Lima de La RoqueRegional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.
Maisa SousaTropical Medicine Center, Federal University of Para, Belém, Brazil.
Viviana OlavarriaCentro de HTLV, Escola Bahiana de Medicina e Saúde Pública, Salvador, Brazil.
Bernardo Galvão-CastroCentro de HTLV, Escola Bahiana de Medicina e Saúde Pública, Salvador, Brazil.
Benedito Antonio Lopes da FonsecaDepartment of Neurology, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.
Augusto César Penalva de OliveiraLaboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.
Jerusa SmidLaboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.
Oswaldo Massaiti TakayanaguiDepartment of Neurology, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.
Jorge CassebLaboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.
Dimas Tadeu CovasRegional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.
Simone KashimaRegional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human T cell lymphotropic virus (HTLV) is the caustive agent of two main conditions i. e., the HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and the adult T-cell leukemia/lymphoma (ATLL). HTLV diagnosis is based on serological and molecular approaches; however, an accurate and validated method is still needed. The objective of this study was to establish a rapid and sensitive molecular test to confirm and discriminate HTLV 1/2 types. The test validation was performed as a multicentric study involving HTLV confirmation centers throughout Brazil. Proviral DNA was extracted from whole blood and the amplification was performed using in-house designed primer and probe sets targeting the

Indexed as

HTLV-1HTLV-2molecular diagnosismultiplex real time PCRreal time PCR

Identifiers

PMID35755037
PMCPMC9218175

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.