ArticleCellular and molecular life sciences : CMLS2022
Single-cell RNA-sequencing of zebrafish hair cells reveals novel genes potentially involved in hearing loss.
Article in Cellular and molecular life sciences : CMLS, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Transcriptomic profiling of inner ear hair cell spontaneous regeneration reveals the key role of dlx5a.Nature communications · 2026Article
- HSD17B7 is required for the function of sensory hair cells by regulating cholesterol synthesis.eLife · 2026Article
- Tekt2 regulates hair cell development and function by maintaining normal kinocilium morphology in zebrafish.Frontiers in cell and developmental biology · 2026Article
- Pvalb8, a Type of Oncomodulin, Regulates Neuromast Development and Auditory Function in Zebrafish.Cells · 2025Article
- Research Progress on MET, Tip Link, and Stereocilia Complex with Special Reference to Zebrafish.International journal of molecular sciences · 2025Review
- Effects of Underwater Noise Exposure on Early Development in Zebrafish.Animals : an open access journal from MDPI · 2025Article
- Single-cell RNA sequencing reveals tissue-specific transcriptomic changes induced by perfluorooctanesulfonic acid (PFOS) in larval zebrafish (Danio rerio).Journal of hazardous materials · 2025Article
- Protective Effects of (-)-Butaclamol Against Gentamicin-Induced Ototoxicity: In Vivo and In Vitro Approaches.International journal of molecular sciences · 2025Article
- Tekt3 Safeguards Proper Functions and Morphology of Neuromast Hair Bundles.International journal of molecular sciences · 2025Article
- The Glutamine Synthetases Are Required for Sensory Hair Cell Formation and Auditory Function in Zebrafish.International journal of molecular sciences · 2024Article
- Transdifferentiation is temporally uncoupled from progenitor pool expansion during hair cell regeneration in the zebrafish inner ear.Development (Cambridge, England) · 2024Article
- Advance and Application of Single-cell Transcriptomics in Auditory Research.Neuroscience bulletin · 2024Review
- Transdifferentiation is uncoupled from progenitor pool expansion during hair cell regeneration in the zebrafish inner ear.bioRxiv : the preprint server for biology · 2024Article
- Spatially resolved single-cell atlas of ascidian endostyle provides insight into the origin of vertebrate pharyngeal organs.Science advances · 2024Article
- Kremen1 regulates the regenerative capacity of support cells and mechanosensory hair cells in the zebrafish lateral line.iScience · 2024Article
- Zebrafish (International journal of molecular sciences · 2023Review
- Organization of vestibular circuits for postural control in zebrafish.Current opinion in neurobiology · 2023Review
- TheInternational journal of molecular sciences · 2023Article
- Loss of ndrg2 Function Is Involved in Notch Activation in Neuromast Hair Cell Regeneration in Zebrafish.Molecular neurobiology · 2023Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
17 authors at 2 institutions in 2 countries.
Funding
Abstract
Hair cells play key roles in hearing and balance, and hair cell loss would result in hearing loss or vestibular dysfunction. Cellular and molecular research in hair cell biology provides us a better understanding of hearing and deafness. Zebrafish, owing to their hair cell-enriched organs, have been widely applied in hair cell-related research worldwide. Similar to mammals, zebrafish have inner ear hair cells. In addition, they also have lateral line neuromast hair cells. These different types of hair cells vary in morphology and function. However, systematic analysis of their molecular characteristics remains lacking. In this study, we analyzed the GFP+ cells isolated from Tg(Brn3c:mGFP) larvae with GFP expression in all hair cells using single-cell RNA-sequencing (scRNA-seq). Three subtypes of hair cells, namely macula hair cell (MHC), crista hair cell (CHC), and neuromast hair cell (NHC), were characterized and validated by whole-mount in situ hybridization analysis of marker genes. The hair cell scRNA-seq data revealed hair cell-specific genes, including hearing loss genes that have been identified in humans and novel genes potentially involved in hair cell formation and function. Two novel genes were discovered to specifically function in NHCs and MHCs, corresponding to their specific expression in NHCs and MHCs. This study allows us to understand the specific genes in hair cell subpopulations of zebrafish, which will shed light on the genetics of both human vestibular and cochlear hair cell function.
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Registered trials
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