Evidence map›Paper›PMID 35752825›Full record

ArticleBMC research notes2022

De novo transcriptome assembly and annotation of the third stage larvae of the zoonotic parasite Anisakis pegreffii.

Marialetizia Palomba, Pietro Libro, Jessica Di Martino, Aurelia Rughetti, Mario Santoro, Simonetta Mattiucci, Tiziana Castrignanò

Open access · goldAbstract read
In one paragraph

Article in BMC research notes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Transcriptomic profile of the zoonotic parasiteFrontiers in cellular and infection microbiology · 2025
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Basophil Activation Test in the Diagnosis ofPathogens (Basel, Switzerland) · 2023
    Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Marialetizia Palomba *Department of Ecological and Biological Sciences, Tuscia University, Viale dell'Università s/n, 01100, Viterbo, Italy.
Pietro Libro *Department of Ecological and Biological Sciences, Tuscia University, Viale dell'Università s/n, 01100, Viterbo, Italy.
Jessica Di MartinoDepartment of Ecological and Biological Sciences, Tuscia University, Viale dell'Università s/n, 01100, Viterbo, Italy.
Aurelia RughettiDepartment of Experimental Medicine, "Sapienza" University of Rome, P.le Aldo Moro, 5, 00185, Rome, Italy.
Mario SantoroDepartment of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Villa Comunale, 1, 80121, Naples, Italy.
Simonetta Mattiucci *Department of Public Health And Infectious Diseases, Section of Parasitology, Sapienza University of Rome, P.le Aldo Moro, 5, 00185, Rome, Italy. simonetta.mattiucci@uniroma1.it.
Tiziana Castrignanò *Department of Ecological and Biological Sciences, Tuscia University, Viale dell'Università s/n, 01100, Viterbo, Italy.
Università degli Studi della Tuscia · ITSapienza University of Rome · ITStazione Zoologica Anton Dohrn · IT

Funding

Ministero della Salute 12367986
6 · The paper itself

Abstract

objectivesAnisakis pegreffii is a zoonotic parasite requiring marine organisms to complete its life-history. Human infection (anisakiasis) occurs when the third stage larvae (L3) are accidentally ingested with raw or undercooked infected fish or squids. A new de novo transcriptome of A. pegreffii was here generated aiming to provide a robust bulk of data to be used for a comprehensive "ready-to-use" resource for detecting functional studies on genes and gene products of A. pegreffii involved in the molecular mechanisms of parasite-host interaction. DATA DESCRIPTION: A RNA-seq library of A. pegreffii L3 was here newly generated by using Illumina TruSeq platform. It was combined with other five RNA-seq datasets previously gathered from L3 of the same species stored in SRA of NCBI. The final dataset was analyzed by launching three assembler programs and two validation tools. The use of a robust pipeline produced a high-confidence protein-coding transcriptome of A. pegreffii. These data represent a more robust and complete transcriptome of this species with respect to the actually existing resources. This is of importance for understanding the involved adaptive and immunomodulatory genes implicated in the "cross talk" between the parasite and its hosts, including the accidental one (humans).

Indexed as

AnisakiasisAnisakisParasitesAnimalsFishesLarvaTranscriptomeAnisakis pegreffiiDe novo assemblyGene annotationTranscriptomeZoonotic parasite

Identifiers

PMID35752825
PMCPMC9233829
OpenAlexW4283447531

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.