ArticleNature communications2022
Wiskott-Aldrich syndrome protein forms nuclear condensates and regulates alternative splicing.
Erratum issuedOpen access · goldAbstract read
In one paragraph
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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14citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
What it found
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Dispensable players: N-WASP and WASP are not crucial for homology-directed DNA repair.EMBO reports · 2026Article
- SRSF9 mediates oncogenic RNA splicing of SLC37A4 via liquid-liquid phase separation to promote oral cancer progression.Journal of advanced research · 2026Article
- Condensatopathies as a mechanistic framework for disease and integrated theranostic intervention.Theranostics · 2026Review
- Alternative Splicing: Molecular Mechanisms, Biological Functions, Diseases, and Potential Therapeutic Targets.MedComm · 2025Review
- Dynamic WNT signaling controls differentiation of hematopoietic progenitor cells from human pluripotent stem cells.Science China. Life sciences · 2025Article
- Nuclear Condensates of WW Domain-Containing Adaptor With Coiled-Coil Regulate Mitophagy via Alternative Splicing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Deciphering a profiling based on multiple post-translational modifications functionally associated regulatory patterns and therapeutic opportunities in human hepatocellular carcinoma.Molecular cancer · 2024Article
- Membraneless organelles in health and disease: exploring the molecular basis, physiological roles and pathological implications.Signal transduction and targeted therapy · 2024Review
- Splicing regulation through biomolecular condensates and membraneless organelles.Nature reviews. Molecular cell biology · 2024Review
- Exploring new roles for RNA-binding proteins in epigenetic and gene regulation.Current opinion in genetics & development · 2024Review
- Proteomic analysis of X-linked dystonia parkinsonism disease striatal neurons reveals altered RNA metabolism and splicing.Neurobiology of disease · 2024Article
- Not too little, not too much: the impact of mutation types in Wiskott-Aldrich syndrome and RAC2 patients.Clinical and experimental immunology · 2023Review
- Biomolecular condensates: Formation mechanisms, biological functions, and therapeutic targets.MedComm · 2023Review
- Correcting inborn errors of immunity: From viral mediated gene addition to gene editing.Seminars in immunology · 2023Review
Corrections and comments
- Erratum issued
Authors and funding
36 authors at 7 institutions in 5 countries.
Baolei Yuan *Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.ORCID http://orcid.org/0000-0003-4159-3319
Xuan Zhou *Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Keiichiro SuzukiGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.ORCID http://orcid.org/0000-0001-5603-9105
Gerardo Ramos-MandujanoBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Mengge WangBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Muhammad TehseenBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Lorena V Cortés-MedinaBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.ORCID http://orcid.org/0000-0001-7718-0730
James J MorescoDepartment of Cell Biology, Scripps Research Institute, La Jolla, CA, 92037, USA.ORCID http://orcid.org/0000-0003-1178-1642
Sarah DunnThe Waitt Advanced Biophotonics Core Facility, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Reyna Hernandez-BenitezGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Tomoaki HishidaGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Na Young KimGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Manal M AndijaniBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Chongwei BiBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.ORCID http://orcid.org/0000-0003-2108-1551
Manching KuNext-generation sequencing core, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Yuta TakahashiGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Jinna XuBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Jinsong QiuDepartment of Cellular & Molecular Medicine, University of California at San Diego, La Jolla, CA, 92093, USA.
Ling HuangIntegrative Genomics and Bioinformatics Core, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Christopher BennerIntegrative Genomics and Bioinformatics Core, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.ORCID http://orcid.org/0000-0002-4618-0719
Emi AizawaGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Jing QuState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.ORCID http://orcid.org/0000-0002-3988-5067
Guang-Hui LiuState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.ORCID http://orcid.org/0000-0001-9289-8177
Zhongwei LiGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.ORCID http://orcid.org/0000-0002-5997-5422
Fei YiGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Yanal GhoshehBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Changwei ShaoDepartment of Cellular & Molecular Medicine, University of California at San Diego, La Jolla, CA, 92093, USA.ORCID http://orcid.org/0000-0002-4082-7200
Maxim ShokhirevIntegrative Genomics and Bioinformatics Core, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Patrizia ComoliPediatric Hematology/Oncology and Cell Factory, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Francesco FrassoniDepartment of Research Laboratories and Director of Center for Stem Cell and Cell Therapy, Instituto G. Gaslini Children Hospital Scientific Institute, 16147, Genova, Italy.
John R YatesDepartment of Cell Biology, Scripps Research Institute, La Jolla, CA, 92037, USA.ORCID http://orcid.org/0000-0001-5267-1672
Xiang-Dong FuDepartment of Cellular & Molecular Medicine, University of California at San Diego, La Jolla, CA, 92093, USA.ORCID http://orcid.org/0000-0001-5499-8732
Concepcion Rodriguez EstebanGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA.
Samir HamdanBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.ORCID http://orcid.org/0000-0001-5192-1852
Juan Carlos Izpisua BelmonteBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia. belmonte@salk.edu.ORCID http://orcid.org/0000-0003-0557-8875
Mo LiBioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia. mo.li@kaust.edu.sa.ORCID http://orcid.org/0000-0003-0827-8907
Salk Institute for Biological Studies · USKing Abdullah University of Science and Technology · SAUniversity of California San Diego · USChinese Academy of Sciences · CNScripps Research Institute · USPoliclinico San Matteo Fondazione · ITUniversity of Southern California · US
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diverse functions of WASP, the deficiency of which causes Wiskott-Aldrich syndrome (WAS), remain poorly defined. We generated three isogenic WAS models using patient induced pluripotent stem cells and genome editing. These models recapitulated WAS phenotypes and revealed that WASP deficiency causes an upregulation of numerous RNA splicing factors and widespread altered splicing. Loss of WASP binding to splicing factor gene promoters frequently leads to aberrant epigenetic activation. WASP interacts with dozens of nuclear speckle constituents and constrains SRSF2 mobility. Using an optogenetic system, we showed that WASP forms phase-separated condensates that encompasses SRSF2, nascent RNA and active Pol II. The role of WASP in gene body condensates is corroborated by ChIPseq and RIPseq. Together our data reveal that WASP is a nexus regulator of RNA splicing that controls the transcription of splicing factors epigenetically and the dynamics of the splicing machinery through liquid-liquid phase separation.
Indexed as
Wiskott-Aldrich SyndromeWiskott-Aldrich Syndrome ProteinAlternative SplicingCell NucleusHumansRNA-Binding ProteinsRNA Polymerase IIRNA Splicing FactorsRNA-Binding ProteinsRNA Polymerase IIRNA Splicing FactorsWiskott-Aldrich Syndrome Protein
Identifiers
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