ArticleNature communications2022
Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children.
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Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15citing papers in PubMed
2.5field-weighted citation impact, top 9% of its field
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Novel Strategies to Profile SARS-CoV-2 and Human Lung Proteome: Inflammatory Pathways in the Spotlight.BioMed research international · 2025Review
- Overcoming bias in estimating epidemiological parameters with realistic history-dependent disease spread dynamics.Nature communications · 2024Article
- Advancing quantitative PCR with color cycle multiplex amplification.Nucleic acids research · 2024Article
- [Results of PCR Pool Testing In Primary and Special Needs Schools In Bavaria For The School Year 2021/2022: Sentinel Surveillance In Face-To-Face Teaching During The Sars-CoV-2 Pandemic].Gesundheitswesen (Bundesverband der Arzte des Offentlichen Gesundheitsdienstes (Germany)) · 2024Article
- Marginal effects of public health measures and COVID-19 disease burden in China: A large-scale modelling study.PLoS computational biology · 2023Article
- Review
- Implementing the Lolli-Method and pooled RT-qPCR testing for SARS-CoV-2 surveillance in schools: a pilot project.Infection · 2023Article
- Towards Point of Care CRISPR-Based Diagnostics: From Method to Device.Journal of functional biomaterials · 2023Review
- Mass testing to end the COVID-19 public health threat.The Lancet regional health. Europe · 2023Article
- SARS-CoV-2 PCR Positivity in Elementary School Classes in Bavaria in the School Year 2021/22—Initial Results From the Evaluation of the Bavarian Pool Test Project in Elementary and Special Schools.Deutsches Arzteblatt international · 2022Article
- Article
- Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children.Nature communications · 2022Article
- Evaluation of efficiency and sensitivity of 1D and 2D sample pooling strategies for SARS-CoV-2 RT-qPCR screening purposes.Scientific reports · 2022Article
- Article
- Detection of asymptomatic SARS-CoV-2 infections in daycare centers, schools, and companies for regional pandemic containment by a PCR testing laboratory cooperative between July 2021 and June 2022.GMS hygiene and infection control · 2022Article
Corrections and comments
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Authors and funding
57 authors at 14 institutions in 2 countries.
Felix Dewald *Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Isabelle Suárez *Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany.
Ronja JohnenCECAD Research center, University of Cologne, Cologne, Germany.ORCID 0000-0002-5150-5266
Jan GrossbachCECAD Research center, University of Cologne, Cologne, Germany.ORCID 0000-0002-9394-5665
Roberto Moran-TovarInstitute for Biological Physics, University of Cologne, Cologne, Germany.ORCID 0000-0002-6291-9376
Gertrud StegerInstitute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Alexander JoachimDepartment of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Gibran Horemheb RubioInstitute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.ORCID 0000-0002-9529-9804
Mira FriesDepartment I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Florian BehrDepartment I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Joao KleyDepartment I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.ORCID 0000-0002-7207-3714
Andreas LingnauMinistry of Schools and Education of North Rhine-Westphalia, Düsseldorf, Germany.
Alina KretschmerDepartment I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.ORCID 0000-0002-6323-1928
Carina GudeCECAD Research center, University of Cologne, Cologne, Germany.
Guadelupe Baeza-FloresCentro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús, Villahermosa, Mexico.
David Laveaga Del ValleCentro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús, Villahermosa, Mexico.
Alberto Roblero-HernandezCentro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús, Villahermosa, Mexico.
Jesus Magana-CerinoCentro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús, Villahermosa, Mexico.
Adriana Torres HernandezBioclilab SA de CV, Villahermosa, Mexico.
Jesus Ruiz-QuinonesCentro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús, Villahermosa, Mexico.ORCID 0000-0002-3723-333X
Konstantin SchegaHealth department of Cologne, Cologne, Germany.ORCID 0000-0002-6297-7947
Viktoria LinneDepartment I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Lena JunkerDepartment I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Marie WunschInstitute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Eva HegerInstitute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Elena KnopsInstitute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Veronica Di CristanzianoInstitute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.ORCID 0000-0003-1604-8386
Meike MeyerDepartment of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Christoph HünselerDepartment of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Lutz T WeberDepartment of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Jan-Christoffer LüersDepartment of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, University of Cologne, Cologne, Germany.ORCID 0000-0002-8273-2234
Gustav QuadeMVZ Labor Dr. Quade & Kollegen GmbH, Cologne, Germany.
Hilmar WisplinghoffLabor Dr. Wisplinghoff, Cologne, Germany.
Carsten TiemannLabor Krone, Bad Salzuflen, Germany.
Rainer ZotzInstitute for Laboratory Medicine ZotzKlimas, Düsseldorf, Germany.
Hassan JomaaSynlab, Leverkusen, Germany.
Arthur PranadaMedizinisches Versorgungszentrum Dr. Eberhard & Partner, Dortmund, Germany.ORCID 0000-0003-2111-9011
Ileana HerzumMedizinische Laboratorien Düsseldorf, Düsseldorf, Germany.
Paul CullenMVZ Labor Münster, Münster, Germany.
Franz-Josef SchmitzMühlenkreiskliniken, Minden, Germany.
Paul PhilipsenLabor Mönchengladbach MVZ Dr. Stein und Kollegen, Mönchengladbach, Germany.
Georg KirchnerEurofins Laborbetriebsgesellschaft Gelsenkirchen GmbH & Eurofins MVZ Medizinisches Labor Gelsenkirchen GmbH, Gelsenkirchen, Germany.
Cornelius KnabbeHeart- and Diabetes Center NRW, Medical Faculty, Ruhr-University Bochum, Institute for Laboratory and Transfusion Medicine, Bad Oeynhausen, Germany.
Martin HellmichInstitute of Medical Statistics and Computational Biology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.ORCID 0000-0001-5174-928X
Michael BuessHealth department of Cologne, Cologne, Germany.
Anna WolffHealth department of Cologne, Cologne, Germany.
Annelene KossowHealth department of Cologne, Cologne, Germany.
Johannes NiessenHealth department of Cologne, Cologne, Germany.
Sebastian JeworutzkiFaculty of Social Science, Ruhr-University Bochum, Bochum, Germany.ORCID 0000-0002-2671-5253
Jörg-Peter SchräplerFaculty of Social Science, Ruhr-University Bochum, Bochum, Germany.
Michael LässigInstitute for Biological Physics, University of Cologne, Cologne, Germany.
Jörg DötschDepartment of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Gerd FätkenheuerCenter for Molecular Medicine Cologne, University of Cologne, Cologne, Germany.
Rolf KaiserInstitute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Andreas Beyer *CECAD Research center, University of Cologne, Cologne, Germany.
Jan Rybniker *Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany.
Florian Klein *Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany. florian.klein@uk-koeln.de.ORCID 0000-0003-1376-1792
University of Cologne · DECBS International Business School · DEHospital Regional de Alta Especialidad del Bajío · MXCenter for HIV and Hepatogastroenterology · DEDüsseldorf University Hospital · DEEurofins (Germany) · DEGerman Institute for Economic Research · DEHeart and Diabetes Center North Rhine-Westphalia · DELaboratory Dr. Wisplinghoff · DELaboratory Krone · DEMedizinisches Versorgungszentrum Prof. Mathey, Prof. Schofer · DERuhr University Bochum · DEStädtische Kliniken Mönchengladbach · DESynlab (Germany) · DE
Funding
No grant is acknowledged in the PubMed record.
Abstract
Systematic SARS-CoV-2 testing is a valuable tool for infection control and surveillance. However, broad application of high sensitive RT-qPCR testing in children is often hampered due to unpleasant sample collection, limited RT-qPCR capacities and high costs. Here, we developed a high-throughput approach ('Lolli-Method') for SARS-CoV-2 detection in children, combining non-invasive sample collection with an RT-qPCR-pool testing strategy. SARS-CoV-2 infections were diagnosed with sensitivities of 100% and 93.9% when viral loads were >10
Indexed as
COVID-19ChildClinical Laboratory TechniquesCOVID-19 TestingHumansSARS-CoV-2Sensitivity and Specificity
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