ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022
In Vivo Click Chemistry Enables Multiplexed Intravital Microscopy.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Toward Realization of Bioorthogonal Chemistry in the Clinic.Topics in current chemistry (Cham) · 2025Review
- FAP-Targeted Fluorescent Imaging Agents to Study Cancer-Associated Fibroblasts In Vivo.Bioconjugate chemistry · 2025Article
- Advances and challenges in precision imaging.The Lancet. Oncology · 2025Review
- Scission-Enhanced Molecular Imaging (SEMI).Bioconjugate chemistry · 2024Article
- Deep learning pipeline for automated cell profiling from cyclic imaging.Scientific reports · 2024Article
- Click Chemistry: Reaction Rates and Their Suitability for Biomedical Applications.Bioconjugate chemistry · 2024Review
- Click chemistry in the development of PROTACs.RSC chemical biology · 2024Review
- Deep Learning Pipeline for Automated Cell Profiling from Cyclic Imaging.Research square · 2023Article
- Highly Active Myeloid Therapy for Cancer.ACS nano · 2023Article
- Bioorthogonal CRISPR/Cas9-Drug Conjugate: A Combinatorial Nanomedicine Platform.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Light-Deactivated Fluorescent Probes (FLASH-Off) for Multiplexed Imaging.Journal of the American Chemical Society · 2023Article
- Oxidative Desymmetrization Enables the Concise Synthesis of a trans-Cyclooctene Linker for Bioorthogonal Bond Cleavage.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- Cycling, Fasting, Fishing, and Other Things: The Emerging World of Biocompatible Chemistries.Advanced biology · 2023Article
- Volume imaging to interrogate cancer cell-tumor microenvironment interactions in space and time.Frontiers in immunology · 2023Review
- In Vivo Click Chemistry Enables Multiplexed Intravital Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The ability to observe cells in live organisms is essential for understanding their function in complex in vivo milieus. A major challenge today has been the limited ability to perform higher multiplexing beyond four to six colors to define cell subtypes in vivo. Here, a click chemistry-based strategy is presented for higher multiplexed in vivo imaging in mouse models. The method uses a scission-accelerated fluorophore exchange (SAFE), which exploits a highly efficient bioorthogonal mechanism to completely remove fluorescent signal from antibody-labeled cells in vivo. It is shown that the SAFE-intravital microscopy imaging method allows 1) in vivo staining of specific cell types in dorsal and cranial window chambers of mice, 2) complete un-staining in minutes, 3) in vivo click chemistries at lower (µm) and thus non-toxic concentrations, and 4) the ability to perform in vivo cyclic imaging. The potential utility of the method is demonstrated by 12 color imaging of immune cells in live mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.