ReviewAmerican journal of physiology. Heart and circulatory physiology2022
Reevaluating methods reporting practices to improve reproducibility: an analysis of methodological rigor for the Langendorff whole heart technique.
Review in American journal of physiology. Heart and circulatory physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Impact of bicarbonate on ionized calcium and cardiac function in albumin-free Langendorff perfusion in hearts of male rats.Physiological reports · 2026Article
- Membrane Oxygenation Improves Functional Myocardial Preservation and Enables Colloid-Enriched Perfusion in the Langendorff Isolated Heart Model.Medical sciences (Basel, Switzerland) · 2026Article
- Improved Rat Heart Preservation Using High-Pressure Gaseous Perfusion with Oxygen-Xenon Mixture.Pathophysiology : the official journal of the International Society for Pathophysiology · 2025Article
- Enhancing outcomes in Langendorff-perfused rodent hearts through perfusion parameter optimization.Scientific reports · 2025Article
- Quantitative assessment of the usability of electromechanical human-based modelling and simulation to replace Langendorff isolated rabbit heart experiments in the preclinical setting.Frontiers in pharmacology · 2025Article
- Crossing the Great Sex-Specific Divide in Cardiac Electrophysiology.JACC. Clinical electrophysiology · 2023Article
- Contractility measurements for cardiotoxicity screening with ventricular myocardial slices of pigs.Cardiovascular research · 2023Article
- Improving rigor and reproducibility in cardiovascular research.American journal of physiology. Heart and circulatory physiology · 2023Article
- When it comes to the heart, age, and sex matter (sometimes).American journal of physiology. Heart and circulatory physiology · 2023Article
- Tipping the scales of understanding: An engineering approach to design and implement whole-body cardiac electrophysiology experimental models.Frontiers in physiology · 2023Article
- The effect of sex and age on ex vivo cardiac electrophysiology: insight from a guinea pig model.American journal of physiology. Heart and circulatory physiology · 2023Article
- Guidelines for assessment of cardiac electrophysiology and arrhythmias in small animals.American journal of physiology. Heart and circulatory physiology · 2022Review
- ATP-Sensitive Potassium Channels in Migraine: Translational Findings and Therapeutic Potential.Cells · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
In recent decades, the scientific community has seen an increased interest in rigor and reproducibility. In 2017, concerns about methodological thoroughness and reporting practices were implicated as significant barriers to reproducibility within the preclinical cardiovascular literature, particularly in studies using animal research. The Langendorff, whole heart technique has proven to be an invaluable research tool, being modified in a myriad of ways to probe questions across the spectrum of physiological and pathophysiological functions of the heart. As a result, significant variability in the application of the Langendorff technique exists. This literature review quantifies the different methods employed in the implementation of the Langendorff technique and provides brief examples of how individual parametric differences can impact the outcomes and interpretation of studies. From 2017 to 2020, significant variability of animal models, anesthesia, cannulation time, perfusate composition, pH, and temperature demonstrate that the technique has diversified to meet new challenges and answer different scientific questions. The review also reveals which individual methods are most frequently reported, even if there is no explicit agreement upon which parameters should be reported. The analysis of methods related to the Langendorff technique suggests a framework for considering methodological approach when interpreting seemingly contradictory results, rather than concluding that results are irreproducible.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.