Evidence map›Paper›PMID 35746973›Full record

ArticleIBRO neuroscience reports2022

Mitochondrial bioenergetics in ocular fibroblasts of two myasthenia gravis cases.

Tarin A Europa, Melissa Nel, Maribanyana R Lebeko, Jeannine M Heckmann

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in IBRO neuroscience reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Tarin A EuropaNeurology Research Group, UCT Neurosciences Institute, University of Cape Town, Cape Town, South Africa.
Melissa NelNeurology Research Group, UCT Neurosciences Institute, University of Cape Town, Cape Town, South Africa.
Maribanyana R LebekoHair and Skin Research Laboratory, Division of Dermatology, Department of Medicine, University of Cape Town, Cape Town, South Africa.
Jeannine M HeckmannNeurology Research Group, UCT Neurosciences Institute, University of Cape Town, Cape Town, South Africa.
University of Cape Town · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Myasthenia gravis (MG) is a rare, treatable, antibody-mediated disease characterized by fatigable muscle weakness of extraocular muscles (EOMs) and non-ocular skeletal muscles. The antibodies are directed against muscle-endplate proteins, most frequently the acetylcholine receptor (AChR) alpha-subunit. Although most MG patients respond to immunosuppressive treatment, some individuals, frequently with African-genetic ancestry, develop treatment-resistant ophthalmoplegia (OP-MG). Although the underlying pathogenetic mechanisms of OP-MG remain unknown, experimental rodent models of MG showed upregulation of genes involved in oxidative metabolism in muscles. EOMs are highly dependent on oxidative metabolism. We opportunistically sampled EOM-tendons of two rare OP-MG patients (and non-MG controls) undergoing re-alignment surgery, and established ocular fibroblast cultures. Metabolic assays were performed on these live cells to assess real-time differences in energy metabolism. To study the cellular bioenergetic profiles in the context of MG, we exposed the cultures to homologous 5% MG sera for 24 h, vs. growth media, from two independent MG patients (with circulating AChR-antibodies) and five controls without MG, and estimated the fold change in oxygen consumption rates in response to three compounds which inhibit different mitochondrial chain complexes. Quantitative PCR (qPCR) was performed in cells before and after MG sera exposure, to assess transcript levels of mitochondrial genes,

Indexed as

AChR, acetylcholine receptorCq, quantitation cycleEAMG, experimental autoimmune myasthenia gravisECAR, extracellular acidification rateEOM, extraocular muscleFCCP, carbonyl cyanide p-trifluoromethoxyphenylhydrazonemax resp, maximal respirationMetabolismMG, myasthenia gravisMitochondriaMyasthenia gravisNMOC, non-mitochondrial oxygen consumptionOCR, oxygen consumption rateOcular fibroblastsOphthalmoplegiaOP-MG, ophthalmoplegic MGOxidative stressPL, proton leakSD, standard deviationSEM, standard error of the meanSeraSMA, smooth muscle actinSRC, spare respiratory capacity

Identifiers

PMID35746973
PMCPMC9210483
OpenAlexW4224254248

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.