ArticleViruses2022
Nationwide Harmonization Effort for Semi-Quantitative Reporting of SARS-CoV-2 PCR Test Results in Belgium.
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Article in Viruses, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Nowcasting epidemic trends using hospital- and community-based virologic test data.Nature communications · 2025Article
- Surveillance of the respiratory syncytial virus outside infancy: impact of testing methods, a retrospective observational study.ERJ open research · 2024Article
- Nationwide quality assurance of high-throughput diagnostic molecular testing during the SARS-CoV-2 pandemic: role of the Belgian National Reference Centre.Virology journal · 2024Review
- Assessment of the clinical and analytical performance of three Seegene Allplex SARS-CoV-2 assays within the VALCOR framework.Microbiology spectrum · 2024Article
- Personal characteristics and transmission dynamics associated with SARS-CoV-2 semi-quantitative PCR test results: an observational study from Belgium, 2021-2022.Frontiers in public health · 2024Observational
- Standardization and Comparison of Emergency Use Authorized COVID-19 Assays and Testing Laboratories.medRxiv : the preprint server for health sciences · 2023Article
- Genomic monitoring of SARS-CoV-2 variants using sentinel SARI hospital surveillance.Influenza and other respiratory viruses · 2023Article
- Minimal requirements for ISO15189 validation and accreditation of three next generation sequencing procedures for SARS-CoV-2 surveillance in clinical setting.Scientific reports · 2023Article
- Converting to an international unit system improves harmonization of results for SARS-CoV-2 quantification: Results from multiple external quality assessments.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2023Article
- Healthcare-Associated SARS-CoV-2 Reinfection after 3 Months with a Phylogenetically Distinct Omicron Variant: A Case Report.Viruses · 2022Article
- Review
- Overview of case definitions and contact tracing indications in the 2022 monkeypox outbreak.Le infezioni in medicina · 2022Review
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Authors and funding
103 authors at 20 institutions in 1 country.
Lize CuypersNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.ORCID 0000-0002-9433-8752
Jannes BodeNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.
Kurt BeuselinckNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.
Lies LaenenNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.ORCID 0000-0001-9118-8608
Klaas DewaeleNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.
Reile JanssenNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.
Arnaud CapronEpidemiology of Infectious Diseases and Quality Service Unit, Scientific Directorate of Epidemiology and Public Health, Sciensano, 1000 Brussels, Belgium.
Yves LafortEpidemiology of Infectious Diseases and Quality Service Unit, Scientific Directorate of Epidemiology and Public Health, Sciensano, 1000 Brussels, Belgium.
Henry ParidaensClinical Laboratory, Centre Hospitalier Régional de la Citadelle, 4000 Liège, Belgium.
Bertrand BearzattoFederal Testing Platform COVID-19, Centre des Technologies Moléculaires Appliquées (CTMA), Institute of Experimental and Clinical Research (IREC), Cliniques Universitaires Saint-Luc and Université Catholique de Louvain (UCLouvain), 1200 Brussels, Belgium.
Mathieu CauchieEurope Hospitals, 1180 Brussels, Belgium.
Aline HuwartClinique André Renard, 4040 Herstal, Belgium.
Jonathan DegosserieFederal Testing Platform COVID-19, Department of Laboratory Medicine, CHU UCL Namur, 5530 Yvoir, Belgium.ORCID 0000-0001-8654-4831
Olivier FagnartSaint-Jean Hospital Laboratory, Cebiodi, 1000 Brussels, Belgium.
Yarah OvermeireMicrobiology, Labo Nuytinck, Anacura, 9940 Evergem, Belgium.
Arlette RouffiangeMicrobiology, Laboratory Luc Olivier, 5380 Fernelmont, Belgium.
Ilse VandecandelaereMicrobiology, Medisch Labo Bruyland, 8500 Kortrijk, Belgium.
Marine DeffontaineLaboratory of Clinical Biology, Centre Hopsitalier de Mouscron, 7700 Mouscron, Belgium.
Thomas PilateClinical Laboratory, Laboratory Medicine, AZ Diest, 3290 Diest, Belgium.ORCID 0000-0001-6586-5679
Nicolas YinDepartment of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), Université de Bruxelles (ULB), 1000 Brussels, Belgium.ORCID 0000-0003-1706-6869
Isabel MicalessiClinical Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium.
Sandrine RoisinMicrobiology, Centre Hospitalier Universitaire de Tivoli, 7100 La Louvière, Belgium.
Veronique MoonsMicrobiology, LKO-LMC Medical Laboratory, 3800 Sint-Truiden, Belgium.
Marijke ReyndersLaboratory Medicine, AZ Sint-Jan Brugge-Oostende AV, 8000 Brugge, Belgium.ORCID 0000-0003-2079-1279
Sophia SteyaertClinical Laboratory, AZ Maria Middelares, 9000 Gent, Belgium.
Coralie HeninFederal Testing Platform COVID-19, Université Libre de Bruxelles, 1070 Brussels, Belgium.ORCID 0000-0002-0210-9371
Elena LazarovaCentre Hospitalier Régional de la Haute Senne, Department of Clinical Biology, 7060 Soignies, Belgium.ORCID 0000-0002-6955-6272
Dagmar ObbelsImelda, Clinical Laboratory, 2820 Bonheiden, Belgium.
François E DufrasneFederal Testing Platform COVID-19, University of Mons, 7000 Mons, Belgium.ORCID 0000-0001-6288-7936
Hendri PirenneSynlab Belgium, Synlab Laboratory Collard, 4020 Liège, Belgium.
Raf SchepersSynlab Belgium, Synlab Laboratory Heppignies, 6220 Heppignies, Belgium.
Anaëlle CollinVivalia, Site Marche-en-Famenne, 6900 Marche, Belgium.
Bruno VerhasseltFederal Testing Platform COVID-19, Department of Laboratory Medicine, Ghent University and Ghent University Hospital, 9000 Gent, Belgium.ORCID 0000-0002-4645-1144
Laurent GilletFederal Testing Platform COVID-19, University of Liège, 4000 Liège, Belgium.
Stijn JonckheereJan Yperman Hospital, Laboratory of Clinical Biology, 8900 Ieper, Belgium.
Philippe Van LintGZA Hospitals, Clinical Laboratory, 2610 Wilrijk, Belgium.
Bea Van den PoelClinical Laboratory, General Hospital Jan Portaels, 1800 Vilvoorde, Belgium.
Yolien Van der BekenMilitary Medicine Lab Capacity, Military Hospital Queen Astrid, 1120 Brussels, Belgium.
Violeta StojkovicCentre Hospitalier Bois de l'Abbaye, Laboratory Service, 4100 Seraing, Belgium.ORCID 0000-0002-3729-938X
Maria-Grazia GarrinoClinical Laboratory, Site Meuse, CHRSM, 5000 Namur, Belgium.
Hannah SegersAZ Glorieux, Clinical Biology, 9600 Ronse, Belgium.
Kevin VosRZ Heilig Hart Tienen, Clinical Biology, 3300 Tienen, Belgium.
Maaike GodefroidLabo Maenhout, 8790 Waregem, Belgium.
Valerie PedeAZ Sint-Elisabeth Zottegem, Laboratory of Clinical Biology, 9600 Zottegem, Belgium.
Friedel NolletBiogazelle NV, Diagnostic Testing, 9052 Zwijnaarde, Belgium.
Vincent ClaesInstitute of Clinical Biology ULB-IBC, 1170 Brussels, Belgium.
Inge VerschraegenAZ St-Blasius Dendermonde, 9200 Dendermonde, Belgium.
Pierre BogaertsCHU UCL Namur, Department of Laboratory Medicine, Molecular Diagnostics Center, 5530 Yvoir, Belgium.
Marjan Van GyselGeneral Hospital Sint-Maria Halle, 1500 Halle, Belgium.
Judith LeursPractimed, 3980 Tessenderlo, Belgium.
Veroniek SaegemanAZ Nikolaas, 9100 Sint-Niklaas, Belgium.
Oriane SoetensDepartment of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, 1090 Brussels, Belgium.
Merijn VanheeClinical Laboratory, Laboratory Medicine, AZ Delta, 8800 Roeselare, Belgium.
Gilberte SchiettekatteCenter for Medical Analysis, 2200 Herentals, Belgium.
Evelyne HuygheZNA Middelheim, Clinical Laboratory, 2020 Antwerp, Belgium.
Steven MartensGeneral Hospital Heilig Hart Lier, 2500 Lier, Belgium.
Ann LemmensAZ Sint-Maarten, Laboratory of Clinical Biology, 2800 Mechelen, Belgium.
Heleen NailisAZ Turnhout, 2300 Turnhout, Belgium.
Kim LaffineurClinique Saint-Luc Bouge, 5004 Namur, Belgium.
Deborah SteenselsClinical Laboratory, Campus Sint-Jan, Hospital Oost-Limburg, 3600 Genk, Belgium.
Elke VanlaereClinical Laboratory, AZ Sint-Lucas Hospital, 9000 Gent, Belgium.ORCID 0000-0002-4567-9999
Jérémie GrasInstitute of Pathology and Genetics, 6041 Gosselies, Belgium.
Gatien RousselClinique Saint Pierre, Laboratory, 1340 Ottignies, Belgium.
Koenraad GijbelsAZ Voorkempen, Clinical Laboratory, 2390 Malle, Belgium.
Michael BoudewijnsClinical Laboratory, Campus Kennedylaan, AZ Groeninge, 8500 Kortrijk, Belgium.
Catherine SionGrand Hôpital de Charleroi, Clinical Biology and Microbiology, 6060 Gilly, Belgium.
Wim AchtergaelClinical Laboratory, Algemeen Stedelijk Ziekenhuis Aalst, 9300 Aalst, Belgium.
Wim MaurissenSint Trudo Hospital, 3800 Sint-Truiden, Belgium.
Luc IlianoLaboratory for Medical Biology Iliano, 9070 Destelbergen, Belgium.
Marianne ChantrenneCHR Verviers, Laboratory of Clinical Biology, 4800 Verviers, Belgium.
Geert VanheuleAZ Rivierenland, Campus Bornem, 2880 Bornem, Belgium.
Reinoud FliesAZ Rivierenland, Campus Rumst, 2840 Rumst, Belgium.
Nicolas HougardyClinical Biology Unit, Vivalia Clinique du Sud-Luxembourg, 6700 Arlon, Belgium.
Mario BerthClinical Laboratory, AZ Alma, 9900 Eeklo, Belgium.ORCID 0000-0003-2233-7213
Vanessa VerbekeMedical Laboratory Medina, 9880 Aalter, Belgium.
Robin MorentDepartment of Laboratory Medicine, Campus Henri Serruys, AZ Sint-Jan Brugge, 8400 Oostende, Belgium.
Anne VankeerberghenLaboratory of Molecular Biology, Campus Aalst-Asse-Ninove, Onze-Lieve-Vrouwziekenhuis, 9300 Aalst, Belgium.
Sébastien BontemsClinical Laboratory, Unit of Clinical Microbiology, CHU Liège, 4000 Liège, Belgium.
Kaat KehoeMicrobiology, Algemeen Medisch Laboratorium, 2020 Antwerp, Belgium.
Anneleen SchallierLaboratory Medical Analysis CRI, 9052 Gent, Belgium.
Giang HoLaboratory, Clinique du MontLégia, Groupe Santé CHC, 4000 Liège, Belgium.
Kristof BafortClinical Laboratory, Mariaziekenhuis Noorderhart, 3900 Pelt, Belgium.
Marijke RaymaekersLaboratory for Molecular Diagnostics, Jessa Hospital, 3500 Hasselt, Belgium.
Yolande PypenMicrobiology, Laboratory Somedi, 2220 Heist-op-den-Berg, Belgium.
Amelie HeinrichsLaboratory of Clinical Biology, Hospital Arlon-Vivalia, 6700 Arlon, Belgium.
Wim SchuermansClinical Laboratory, Ziekenhuis Geel, 2440 Geel, Belgium.ORCID 0000-0001-8613-2394
Dominique CuigniezMicrobiology, Medilab, 9000 Gent, Belgium.
Salah Eddine LaliClinical Laboratory, CHU Charleroi, 6042 Charleroi, Belgium.
Stefanie DriegheMicrobiology, Algemeen Medisch Laboratorium West, 8850 Ardooie, Belgium.
Dieter OryClinical Laboratory, Heilig Hart Ziekenhuis Mol, 2400 Mol, Belgium.
Marie Le MercierFederal Testing Platform COVID-19, University Hospitals Antwerp, 2650 Edegem, Belgium.ORCID 0000-0002-1338-4360
Kristel Van LaethemFederal Testing Platform COVID-19, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.ORCID 0000-0001-6036-2271
Inge ThoelenClinical Laboratory, AZ Vesalius Tongeren, 3700 Tongeren, Belgium.
Sarah VandammeMicrobiology Laboratory, University Hospitals Antwerp, 2650 Edegem, Belgium.
Iqbal MansoorClinical Laboratory, Hospital Hornu Epicura, 7301 Boussu, Belgium.
Carl VaelClinical Laboratory, AZ Klina, 2930 Brasschaat, Belgium.
Maxime De SloovereLaboratory Clinical Biology, Ziekenhuis Waregem, 8790 Waregem, Belgium.ORCID 0000-0001-7710-2302
Katrien DeclerckMicrobiology, Eurofins Labo Van Poucke, 8500 Kortrijk, Belgium.
Elisabeth DequekerNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.
Stefanie DesmetNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.ORCID 0000-0002-4338-1929
Piet MaesLaboratory of Clinical and Epidemiological Virology, Department of Microbiology, Rega Institute for Medical Research, Immunology and Transplantation, KU Leuven, 3000 Leuven, Belgium.
Katrien LagrouNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.ORCID 0000-0001-8668-1350
Emmanuel AndréNational Reference Centre for Respiratory Pathogens, Department of Laboratory Medicine, University Hospitals Leuven, 3000 Leuven, Belgium.ORCID 0000-0001-8321-3770
KU Leuven · BEAZ Sint-Jan · BEAZ Sint-Lucas · BEAntwerp University Hospital · BEAZ Sint-Blasius · BECHU Dinant Godinne UCL Namur · BEGrand Charleroi Hospital · BEOnze Lieve Vrouwziekenhuis Hospital · BERega Institute for Medical Research · BERenson (Belgium) · BESciensano (Belgium) · BEUniversité Libre de Bruxelles · BEUniversity of Liège · BEAlma · BEAZ Delta · BEAZ Groeninge · BEAZ Klina · BEAZ Maria Middelares · BEAZ Turnhout · BECentre Hospitalier Chrétien · BE
Funding
No grant is acknowledged in the PubMed record.
Abstract
From early 2020, a high demand for SARS-CoV-2 tests was driven by several testing indications, including asymptomatic cases, resulting in the massive roll-out of PCR assays to combat the pandemic. Considering the dynamic of viral shedding during the course of infection, the demand to report cycle threshold (Ct) values rapidly emerged. As Ct values can be affected by a number of factors, we considered that harmonization of semi-quantitative PCR results across laboratories would avoid potential divergent interpretations, particularly in the absence of clinical or serological information. A proposal to harmonize reporting of test results was drafted by the National Reference Centre (NRC) UZ/KU Leuven, distinguishing four categories of positivity based on RNA copies/mL. Pre-quantified control material was shipped to 124 laboratories with instructions to setup a standard curve to define thresholds per assay. For each assay, the mean Ct value and corresponding standard deviation was calculated per target gene, for the three concentrations (10
Indexed as
COVID-19SARS-CoV-2BelgiumHumansPandemicsReal-Time Polymerase Chain ReactioninfectivityPCRRNA copies/mLSARS-CoV-2semi-quantitative reporting
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