Evidence map›Paper›PMID 35745526›Full record

ArticlePathogens (Basel, Switzerland)2022

Utilization of Recombinant Baculovirus Expression System to Produce the RBD Domain of SARS-CoV-2 Spike Protein.

Youpeng Fan, Junhong Wei, Wei Wang, Chunfeng Li, Guoqing Pan, Timothy Keiffer, Jialing Bao, Zeyang Zhou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Youpeng FanState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Junhong WeiState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.ORCID 0000-0002-5748-3977
Wei WangState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Chunfeng LiState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Guoqing PanState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Timothy KeifferDepartment of Microbiology and Immunology, Center for Molecular and Tumor Virology, Feist-Weiller Cancer Center, Louisiana State University, Baton Rouge, LA 71130, USA.
Jialing BaoState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Zeyang ZhouState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.ORCID 0000-0003-2296-0742
Southwest University · CNChongqing Normal University · CNLouisiana State University · US

Funding

Academician Fund of Chongqing/Natural Science Foundation of Chongqing cstc2018jcyj-yszxX0012
6 · The paper itself

Abstract

Continuous outbreaks of viral diseases in humans facilitates a need for the rapid development of viral test kits and vaccines. These require expression systems to produce a pure and high yield of target viral proteins. We utilized a baculovirus-silkworm expression system to produce the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. First, we had to develop a strategy for constructing a recombinant baculovirus for RBD expression. For this, the coding region of the

Indexed as

baculovirusBmCPVBmNPVCOVID-19 spike proteinRBD

Identifiers

PMID35745526
PMCPMC9228350
OpenAlexW4282839330

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.