ArticleMolecules (Basel, Switzerland)2022
Precise Quantification of Molybdate In Vitro by the FRET-Based Nanosensor 'MolyProbe'.
Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- The Final Step in Molybdenum Cofactor Biosynthesis-A Historical View.Molecules (Basel, Switzerland) · 2024Review
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Monitoring nutrients in plants with genetically encoded sensors: achievements and perspectives.Plant physiology · 2023Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Molybdenum (Mo) is an essential trace element in all kingdoms of life. Mo is bioavailable as the oxyanion molybdate and gains biological activity in eukaryotes when bound to molybdopterin, forming the molybdenum cofactor. The imbalance of molybdate homeostasis results in growth deficiencies or toxic symptoms within plants, fungi and animals. Recently, fluorescence resonance energy transfer (FRET) methods have emerged, monitoring cellular and subcellular molybdate distribution dynamics using a genetically encoded molybdate-specific FRET nanosensor, named MolyProbe. Here, we show that the MolyProbe system is a fast and reliable in vitro assay for quantitative molybdate determination. We added a Strep-TagII affinity tag to the MolyProbe protein for quick and easy purification. This MolyProbe is highly stable, resistant to freezing and can be stored for several weeks at 4 °C. Furthermore, the molybdate sensitivity of the assay peaked at low nM levels. Additionally, The MolyProbe was applied in vitro for quantitative molybdate determination in cell extracts of the plant
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