ArticleMicroorganisms2022
Comparison of DNA Extraction and Amplification Techniques for Use with Engorged Hard-Bodied Ticks.
Article in Microorganisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Establishment and application of a multiplex qPCR panel for multi-host surveillance of tick-Borne pathogens.Emerging microbes & infections · 2026Article
- Vector-borne pathogen profiling and phylogeny of ticks infesting domestic dogs in Chad, Africa, using targeted next-generation sequencing and DNA barcoding.Parasites & vectors · 2026Article
- Combining PCR and Metagenomic Approaches to Reveal Tick-Borne Pathogens in Ticks Collected from Livestock and Companion Animals in Cambodia.Pathogens (Basel, Switzerland) · 2026Article
- Molecular epidemiology and phylogenetic analysis of Anaplasma ovis and Anaplasma marginale in Ixodidae infesting livestock in northwestern Iran.BMC veterinary research · 2026Article
- Case Report: Retrospective discovery ofFrontiers in veterinary science · 2026Article
- Community-engaged passive tick surveillance highlights the diversity of ticks and tick-borne pathogens in Illinois.PloS one · 2026Article
- Harnessing 50 years of tick population genetics: Choosing the right molecular tool for contemporary research.The Journal of animal ecology · 2025Review
- Microfluidic Sensors Integrated with Smartphones for Applications in Forensics, Agriculture, and Environmental Monitoring.Micromachines · 2025Review
- Article
- Sanitation in urban areas may limit the spread of antimicrobial resistance via flies.PloS one · 2024Article
- Update on tick-borne pathogens detection methods within ticks.Current research in parasitology & vector-borne diseases · 2024Article
- Amplicon-Based High-Throughput Sequencing Method for Genotypic Characterization of Norovirus in Oysters.Applied and environmental microbiology · 2023Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Tick-borne infections are a serious threat to humans, livestock, and companion animals in many parts of the world, often leading to high morbidity and mortality rates, along with decreased production values and/or costly treatments. The prevalence of the microbes responsible for these infections is typically assessed by the molecular identification of pathogens within the tick vectors. Ticks sampled from animals are often engorged with animal blood, presenting difficulties in the amplification of nucleic acids due to the inhibitory effects of mammalian blood on the enzymes used in polymerase chain reactions (PCRs). This study tested two tick preparation methods, three methods of DNA extraction, and four commercially available DNA polymerases to determine the most reliable method of extracting and amplifying DNA from engorged ticks. Our study found that the phenol-chloroform extraction method yielded the highest concentration of DNA, yet DNA extracted by this method was amplified the least successfully. Thermo Scientific's Phusion Plus PCR Master Mix was the best at amplifying the tick 16s rRNA gene, regardless of extraction method. Finally, our study identified that using the Qiagen DNeasy Blood & Tissues kit for DNA extraction coupled with either Phusion Plus PCR Master Mix or GoTaq DNA polymerase Master Mix is the best combination for the optimized amplification of DNA extracted from engorged ticks.
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Registered trials
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