Evidence map›Paper›PMID 35744483›Full record

ArticleMicromachines2022

A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device.

Chengzhuang Yu, Shanshan Li, Chunyang Wei, Shijie Dai, Xinyi Liang, Junwei Li

Open access · goldAbstract read
In one paragraph

Article in Micromachines, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. A Guide to Biodetection in Droplets.Analytical chemistry · 2024
    Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Chengzhuang YuHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, China.
Shanshan LiHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, China.ORCID 0000-0001-8293-9676
Chunyang WeiHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, China.
Shijie DaiState Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300130, China.
Xinyi LiangInstitute of Biophysics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300130, China.
Junwei LiInstitute of Biophysics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300130, China.ORCID 0000-0002-1724-4752
Hebei University of Technology · CN

Funding

Department of Human Resources and Social Security of Hebei Province C20200314 and C20210337Fund for Distinguish Young Scholars in Tianjin 2018 3rd Round 180191Hebei Natural Science Foundation E2022202127, E2020202101 and F2021202001Hebei Science and Technology Foundation 19271707DJiangsu Key Laboratory of Advanced Manufacturing Equipment and Technology FMZ202016National Natural Science Foundation of China 51728502
6 · The paper itself

Abstract

A fluorescence microscope is one of the most important tools for biomedical research and laboratory diagnosis. However, its high cost and bulky size hinder the application of laboratory microscopes in space-limited and low-resource applications. Here, in this work, we proposed a portable and cost-effective fluorescence microscope. Assembled from a set of 3D print components and a webcam, it consists of a three-degree-of-freedom sliding platform and a microscopic imaging system. The microscope is capable of bright-field and fluorescence imaging with micron-level resolution. The resolution and field of view of the microscope were evaluated. Compared with a laboratory-grade inverted fluorescence microscope, the portable microscope shows satisfactory performance, both in the bright-field and fluorescence mode. From the configurations of local resources, the microscope costs around USD 100 to assemble. To demonstrate the capability of the portable fluorescence microscope, we proposed a quantitative polymerase chain reaction experiment for meat product authenticating applications. The portable and low-cost microscope platform demonstrates the benefits in space-constrained environments and shows high potential in telemedicine, point-of-care testing, and more.

Indexed as

fluorescencemeat products authenticatingmicroscopeportable

Identifiers

PMID35744483
PMCPMC9227208
OpenAlexW4281635443

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.