Integration of Adenylate Kinase 1 with Its Peptide Conformational Imprint.

Cheng-Hsin Wu, Chung-Yin Lin, Tzu-Chieh Lin, Dar-Fu Tai

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Cheng-Hsin WuDepartment of Chemistry, National Dong Hwa University, Hualien 97403, Taiwan.

Chung-Yin LinMedical Imaging Research Center, Institute for Radiological Research, Chang Gung University, Taoyuan 33302, Taiwan.

Tzu-Chieh LinDepartment of Chemistry, National Dong Hwa University, Hualien 97403, Taiwan.

Dar-Fu TaiDepartment of Chemistry, National Dong Hwa University, Hualien 97403, Taiwan.

Funding

The Ministry of Science and Technology (MOST) Taiwan 100-213-M-259-001-MY3The Ministry of Science and Technology (MOST) Taiwan 109-221-E-182-008-MY3

6 · The paper itself

Abstract

In the present study, molecularly imprinted polymers (MIPs) were used as a tool to grasp a targeted α-helix or β-sheet of protein. During the fabrication of the hinge-mediated MIPs, elegant cavities took shape in a special solvent on quartz crystal microbalance (QCM) chips. The cavities, which were complementary to the protein secondary structure, acted as a peptide conformational imprint (PCI) for adenylate kinase 1 (AK1). We established a promising strategy to examine the binding affinities of human AK1 in conformational dynamics using the peptide-imprinting method. Moreover, when bound to AK1, PCIs are able to gain stability and tend to maintain higher catalytic activities than free AK1. Such designed fixations not only act on hinges as accelerators; some are also inhibitors. One example of PCI inhibition of AK1 catalytic activity takes place when PCI integrates with an AK1

Indexed as

Molecular ImprintingAdenylate KinaseHumansPeptidesProteinsQuartz Crystal Microbalance TechniquesAdenylate Kinaseadenylate kinase 1PeptidesProteinsadenylate kinase 1conformationinhibitionmolecularly imprinted polymerssecondary structure

Identifiers

PMID35742970

DOI10.3390/ijms23126521

PMCPMC9223553

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.