Evidence map›Paper›PMID 35741889›Full record

ArticleFoods (Basel, Switzerland)2022

Transcriptome-Guided Identification of Pectin Methyl-Esterase-Related Enzymes and Novel Molecular Processes Effectuating the Hard-to-Cook Defect in Common Bean (

Mary Esther Muyoka Toili, Ramon de Koning, Raphaël Kiekens, Nelson Ndumba, Samuel Wahome, Sylvester Anami, Stephen Mwangi Githiri, Geert Angenon

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Foods (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mary Esther Muyoka ToiliLaboratory of Plant Genetics, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, 1050 Brussels, Belgium.ORCID 0000-0002-6101-0857
Ramon de KoningLaboratory of Plant Genetics, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, 1050 Brussels, Belgium.ORCID 0000-0002-0001-9911
Raphaël KiekensLaboratory of Plant Genetics, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, 1050 Brussels, Belgium.ORCID 0000-0003-2749-3397
Nelson NdumbaDepartment of Biochemistry, School of Biomedical Sciences, College of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-00200, Kenya.
Samuel WahomeDepartment of Horticulture and Food Security, School of Agriculture and Environmental Sciences, College of Agriculture and Natural Resources, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-00200, Kenya.
Sylvester AnamiInstitute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-00200, Kenya.
Stephen Mwangi GithiriDepartment of Horticulture and Food Security, School of Agriculture and Environmental Sciences, College of Agriculture and Natural Resources, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-00200, Kenya.
Geert AngenonLaboratory of Plant Genetics, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, 1050 Brussels, Belgium.ORCID 0000-0001-8177-4961

Funding

VLIR-UOS KE2017IUC037A101
6 · The paper itself

Abstract

The hard-to-cook defect in common beans is dictated by the ability to achieve cell separation during cooking. Hydrolysis of pectin methyl-esters by the pectin methyl-esterase (PME) enzyme influences cell separation. However, the contributions of the PME enzyme and the cell wall to the hard-to-cook defect have not been studied using molecular tools. We compared relevant molecular processes in fast- and slow-cooking bean varieties to understand the mechanisms underpinning the hard-to-cook defect. A PME spectrophotometric assay showed minor differences in enzyme activity between varieties. Meanwhile, a PME

Indexed as

hard-to-cook defectpectin methyl-esterase inhibitor (PMEI)pectin methyl-esterase (PME)Phaseolus vulgarisRNA sequencing

Identifiers

PMID35741889
PMCPMC9222787

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.