Evidence map›Paper›PMID 35740912›Full record

ArticleBiomolecules2022

OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels.

Thomas J Kleist, I Winnie Lin, Sophia Xu, Grigory Maksaev, Mayuri Sadoine, Elizabeth S Haswell, Wolf B Frommer, Michael M Wudick

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Thomas J KleistInstitute for Molecular Physiology, Heinrich Heine University, 40225 Düsseldorf, Germany.ORCID 0000-0003-2568-7323
I Winnie LinDepartment of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA.
Sophia XuDepartment of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA.
Grigory MaksaevNSF Center for Engineering Mechanobiology, Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA.
Mayuri SadoineInstitute for Molecular Physiology, Heinrich Heine University, 40225 Düsseldorf, Germany.ORCID 0000-0003-0217-6731
Elizabeth S HaswellNSF Center for Engineering Mechanobiology, Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA.ORCID 0000-0002-4246-065X
Wolf B FrommerInstitute for Molecular Physiology, Heinrich Heine University, 40225 Düsseldorf, Germany.
Michael M WudickInstitute for Molecular Physiology, Heinrich Heine University, 40225 Düsseldorf, Germany.ORCID 0000-0002-4332-369X

Funding

Biophysical, Structural and Functional Analysis of Mechanosensitive ChannelsR01GM084211 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI HASWELL, ELIZABETH S, PHILLIPS, ROB · 2008 to 2016
$3.6M
NIH HHS 2R01GM084211
6 · The paper itself

Abstract

Interactions between physical forces and membrane proteins underpin many forms of environmental sensation and acclimation. Microbes survive osmotic stresses with the help of mechanically gated ion channels and osmolyte transporters. Plant mechanosensitive ion channels have been shown to function in defense signaling. Here, we engineered genetically encoded osmolality sensors (OzTracs) by fusing fluorescent protein spectral variants to the mechanosensitive ion channels MscL from

Indexed as

Arabidopsis ProteinsEscherichia coli ProteinsIon ChannelsMembrane ProteinsArabidopsisEscherichia coliOsmolar ConcentrationOsmotic PressureArabidopsis ProteinsEscherichia coli ProteinsIon ChannelsMembrane ProteinsMscL protein, E coliMSL10 protein, Arabidopsismechanosensitive proteinosmolalitysensoryeast

Identifiers

PMID35740912
PMCPMC9221499

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.