Evidence map›Paper›PMID 35739829›Full record

ArticleAnimals : an open access journal from MDPI2022

Frequency of Semen Collection Affects Ram Sperm Cryoresistance.

Cristina Palacin-Martinez, Mercedes Alvarez, Rafael Montes-Garrido, Marta Neila-Montero, Luis Anel-Lopez, Paulino de Paz, Luis Anel, Marta F Riesco

Open access · goldAbstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Cristina Palacin-MartinezAssisted Reproduction Techniques Research Group (Itra-ULE), INDEGSAL, University of León, 24071 León, Spain.ORCID 0000-0001-7901-228X
Mercedes AlvarezAssisted Reproduction Techniques Research Group (Itra-ULE), INDEGSAL, University of León, 24071 León, Spain.ORCID 0000-0002-9057-4758
Rafael Montes-GarridoAssisted Reproduction Techniques Research Group (Itra-ULE), INDEGSAL, University of León, 24071 León, Spain.ORCID 0000-0003-4000-7867
Marta Neila-MonteroAssisted Reproduction Techniques Research Group (Itra-ULE), INDEGSAL, University of León, 24071 León, Spain.ORCID 0000-0003-2180-9434
Luis Anel-LopezAssisted Reproduction Techniques Research Group (Itra-ULE), INDEGSAL, University of León, 24071 León, Spain.ORCID 0000-0001-7179-5742
Paulino de PazAssisted Reproduction Techniques Research Group (Itra-ULE), INDEGSAL, University of León, 24071 León, Spain.ORCID 0000-0003-2531-595X
Luis AnelAssisted Reproduction Techniques Research Group (Itra-ULE), INDEGSAL, University of León, 24071 León, Spain.
Marta F RiescoAssisted Reproduction Techniques Research Group (Itra-ULE), INDEGSAL, University of León, 24071 León, Spain.ORCID 0000-0002-3655-6401
Universidad de León · ES

Funding

Junta de Castilla y León LE253P18Junta de Castilla y León ORDEN EDU/556/2019MEC FPU17/04142Ministry of Economy, Industry and Competitiveness AGL2017-83098-RMinistry of Economy, Industry and Competitiveness PRE2018-086400
6 · The paper itself

Abstract

The improvement of frozen-thawed sperm quality has been mostly approached from the view of cryopreservation protocol optimization in terms of cryoprotectant solutions, freezing-thawing rates and antioxidant supplementation, while the impact of sperm collection frequency remains unknown in rams. In this work, a multiparametric study was carried out in cooled and frozen-thawed semen to evaluate sperm quality after different semen collection frequencies during a month: zero sperm collection (0 CW), four sperm collections per week (4 CW), and ten sperm collections per week (10 CW). Traditional analyses have been applied, in combination with novel technologies related to redox balance. Frozen-thawed semen quality showed a significant decrease (p < 0.05) in 0 CW and 10 CW in comparison to 4 CW, concerning motility and kinetics parameters. However, apoptosis showed a significant increase (p < 0.05) in 10 CW in comparison to 0 CW and 4 CW. The employment methods related to redox balance provided us with the definitive probe to ensure the influence of collection frequency on balance redox after thawing. Specifically, glutathione peroxidase (GPX) and superoxide dismutase (SOD) activity showed a significant decrease (p < 0.05) in 10 CW compared to 0 CW and 4 CW. The characterization of alternative strategies to sperm cryopreservation based on consideration of male sexual regimes, could improve the quality of frozen-thawed sperm.

Indexed as

GPX activityramsexual regimeSOD activitysperm cryopreservation

Identifiers

PMID35739829
PMCPMC9219472
OpenAlexW4281691828

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.