Evidence map›Paper›PMID 35739346›Full record

ArticleApplied microbiology and biotechnology2022

Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4.

Adelaide Braga, Daniela Gomes, João Rainha, Beatriz B Cardoso, Cláudia Amorim, Sara C Silvério, María Fernández-Lobato, Joana L Rodrigues, Lígia R Rodrigues

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Adelaide BragaCEB-Center of Biological Engineering, Universidade Do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.ORCID http://orcid.org/0000-0001-7432-0726
Daniela GomesCEB-Center of Biological Engineering, Universidade Do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.ORCID http://orcid.org/0000-0001-7703-2391
João RainhaCEB-Center of Biological Engineering, Universidade Do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.ORCID http://orcid.org/0000-0003-3963-234X
Beatriz B CardosoCEB-Center of Biological Engineering, Universidade Do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.ORCID http://orcid.org/0000-0003-3230-7659
Cláudia AmorimCEB-Center of Biological Engineering, Universidade Do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.ORCID http://orcid.org/0000-0002-0999-0405
Sara C SilvérioCEB-Center of Biological Engineering, Universidade Do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.ORCID http://orcid.org/0000-0003-3541-3908
María Fernández-LobatoDepartment of Crystallography and Structural Biology, Institute of Physical Chemistry-Rocasolano (CSIC), 28006, Madrid, Spain.ORCID http://orcid.org/0000-0003-0778-7443
Joana L RodriguesCEB-Center of Biological Engineering, Universidade Do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.ORCID http://orcid.org/0000-0002-3217-2320
Lígia R RodriguesCEB-Center of Biological Engineering, Universidade Do Minho, Campus de Gualtar, 4710-057, Braga, Portugal. lrmr@deb.uminho.pt.ORCID http://orcid.org/0000-0001-9265-0630

Funding

Fundação para a Ciência e a Tecnologia POCI-01-0145-FEDER-029549Fundação para a Ciência e a Tecnologia SFRH/BD/04433/2020Fundação para a Ciência e a Tecnologia SFRH/BD/132324/2017Fundação para a Ciência e a Tecnologia SFRH/BD/138325/2018Fundação para a Ciência e a Tecnologia UIDB/04469/2020Fundação para a Ciência e a Tecnologia UMINHO/BPD/4/2019
6 · The paper itself

Abstract

Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated β-fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB that was able to produce 73.4 ± 1.6 g L

Indexed as

ZymomonasEthanolFermentationOligosaccharidesSucroseEthanolfructooligosaccharideOligosaccharidesSucrose6-kestoseFructooligosaccharides (FOS)Levansucrase (sacB)One-step fermentationSchwanniomyces occidentalis (Ffase-Leu196)Zymomonas mobilis

Identifiers

PMID35739346

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.