ArticleNature methods2022
Multiplexed bioluminescence microscopy via phasor analysis.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- An Open, Integrated Platform for Multiplexed Bioluminescence Microscopy.Chemical & biomedical imaging · 2026Article
- Multimodal Phasor Analysis for Digital Pathology: Quantitative Characterization of Liver Iron Overload.Chemical & biomedical imaging · 2026Article
- Expanded applications of bioluminescence microscopy with phasor analysis.Cell reports methods · 2026Article
- A Modular Platform for Quantitative Two-Color Bioluminescence Combining NanoLuc and Red-Shifted NanoPrism Luciferases.ACS chemical biology · 2026Article
- Bioluminescent Probes for Multiplexed RNA Imaging.Journal of the American Chemical Society · 2026Article
- High-Throughput Hyperspectral and Multiplexed Super-Resolution Fluorescence Imaging by SP-STORM.JACS Au · 2026Article
- Redefining colocalization analysis with a novel phasor mixing coefficient.Journal of cell science · 2026Article
- Semisynthetic, Multicolor Probes for Bioluminescence Imaging.ACS omega · 2025Article
- Delineation of gastrointestinal tumors biopsies using a fluorescence lifetime imaging optical fiber probe.Journal of biophotonics · 2025Article
- ESPRESSO: spatiotemporal omics based on organelle phenotyping.Nature methods · 2025Article
- Spectral phasor imaging on a commercial confocal microscope without a spectral detector.Scientific reports · 2025Article
- A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging.Nature methods · 2025Article
- High-Speed Hyperspectral Imaging for Near Infrared Fluorescence and Environmental Monitoring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Large-scale combinatorial optical barcoding of cells with laser particles.Light, science & applications · 2025Article
- GSLab: open-source platform for advanced phasor analysis in fluorescence microscopy.Bioinformatics (Oxford, England) · 2025Article
- GSLab: Open-Source Platform for Advanced Phasor Analysis in Fluorescence Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Illuminating anions in biology with genetically encoded fluorescent biosensors.Current opinion in chemical biology · 2025Review
- Creating coveted bioluminescence colors for simultaneous multi-color bioimaging.Science advances · 2025Article
- Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis.Chemical & biomedical imaging · 2024Article
- Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
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Authors and funding
11 authors.
Funding
Abstract
Bioluminescence imaging with luciferase-luciferin pairs is a well-established technique for visualizing biological processes across tissues and whole organisms. Applications at the microscale, by contrast, have been hindered by a lack of detection platforms and easily resolved probes. We addressed this limitation by combining bioluminescence with phasor analysis, a method commonly used to distinguish spectrally similar fluorophores. We built a camera-based microscope equipped with special optical filters to directly assign phasor locations to unique luciferase-luciferin pairs. Six bioluminescent reporters were easily resolved in live cells, and the readouts were quantitative and instantaneous. Multiplexed imaging was also performed over extended time periods. Bioluminescent phasor further provided direct measures of resonance energy transfer in single cells, setting the stage for dynamic measures of cellular and molecular features. The merger of bioluminescence with phasor analysis fills a long-standing void in imaging capabilities, and will bolster future efforts to visualize biological events in real time and over multiple length scales.
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