Evidence map›Paper›PMID 35739142›Full record

ArticleScientific reports2022

Fibrillin-1 mutant mouse captures defining features of human primary open glaucoma including anomalous aqueous humor TGF beta-2.

MinHee K Ko, Jeong-Im Woo, Jose M Gonzalez, Gayeoun Kim, Lynn Sakai, Janos Peti-Peterdi, Jonathan A Kelber, Young-Kwon Hong, James C Tan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

MinHee K KoDoheny Eye Institute, Los Angeles, CA, USA.
Jeong-Im WooDoheny Eye Institute, Los Angeles, CA, USA.
Jose M GonzalezDoheny Eye Institute, Los Angeles, CA, USA.
Gayeoun KimDoheny Eye Institute, Los Angeles, CA, USA.
Lynn SakaiDepartment of Medical and Molecular Genetics, Oregon Health Sciences University, Portland, OR, USA.
Janos Peti-PeterdiDepartments of Physiology, Biophysics and Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Jonathan A KelberDevelopmental Oncogene Laboratory, California State University Northridge, Northridge, CA, USA.
Young-Kwon HongDepartment of Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
James C TanDoheny Eye Institute, Los Angeles, CA, USA. oranghutan@aol.com.
Doheny Eye Institute · USUniversity of Southern California · USCalifornia State University, Northridge · USOregon Health & Science University · USUniversity of California, Los Angeles · US

Funding

SPATIOTEMPORAL MECHANISMS OF EIF5A1/2-MEDIATED METASTASIS IN TRIPLE-NEGATIVE BREAST CANCERSC1GM121182 · NIGMS · CALIFORNIA STATE UNIVERSITY NORTHRIDGE · PI KELBER, JONATHAN · 2017 to 2024
$3.2M
Fibrillin-1 and TGFB2 Abnormality Models POAG Pathogenesis and TreatmentR01EY026935 · NEI · SIGHTGENE, INC. · PI TAN, CHEE HIAN · 2017 to 2019
$1.2M
Multiphoton microscope replacement for USC shared resourceS10OD021833 · OD · UNIVERSITY OF SOUTHERN CALIFORNIA · PI PETI-PETERDI, JANOS · 2017 to 2017
$600k
Flow Dependent Development and Maintenance of Schlemm's CanalR21EY026260 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI HONG, YOUNG-KWON · 2016 to 2017
$454k
Contractile Modulation of Distal Aqueous Humor DrainageR21EY029062 · NEI · SIGHTGENE, INC. · PI TAN, CHEE HIAN · 2018 to 2019
$432k
NEI NIH HHS R01 EY026935NEI NIH HHS R21 EY026260NEI NIH HHS R21 EY029062NIGMS NIH HHS SC1 GM121182NIH HHS 1R01EY026935NIH HHS 2SC1GM121182-05NIH HHS R21EY026260NIH HHS S10 OD021833NIH HHS S10OD021833
6 · The paper itself

Abstract

Primary open angle glaucoma (POAG) features an optic neuropathy, elevated aqueous humor (AH) TGFβ2, and major risk factors of central corneal thickness (CCT), increasing age and intraocular pressure (IOP). We examined Tight skin (Tsk) mice to see if mutation of fibrillin-1, a repository for latent TGFβ, is associated with characteristics of human POAG. We measured: CCT by ocular coherence tomography (OCT); IOP; retinal ganglion cell (RGC) and optic nerve axon counts by microscopic techniques; visual electrophysiologic scotopic threshold responses (STR) and pattern electroretinogram (PERG); and AH TGFβ2 levels and activity by ELISA and MINK epithelial cell-based assays respectively. Tsk mice had open anterior chamber angles and compared with age-matched wild type (WT) mice: 23% thinner CCT (p < 0.003); IOP that was higher (p < 0.0001), more asymmetric (p = 0.047), rose with age (p = 0.04) and had a POAG-like frequency distribution. Tsk mice also had RGCs that were fewer (p < 0.04), declined with age (p = 0.0003) and showed increased apoptosis and glial activity; fewer optic nerve axons (p = 0.02); abnormal axons and glia; reduced STR (p < 0.002) and PERG (p < 0.007) visual responses; and higher AH TGFβ2 levels (p = 0.0002) and activity (p = 1E-11) especially with age. Tsk mice showed defining features of POAG, implicating aberrant fibrillin-1 homeostasis as a pathogenic contributor to emergence of a POAG phenotype.

Indexed as

Aqueous HumorFibrillin-1Glaucoma, Open-AngleAdipokinesAnimalsHumansIntraocular PressureMiceRetinal Ganglion CellsTonometry, OcularTransforming Growth Factor beta2AdipokinesFbn1 protein, mouseFibrillin-1Tgfb2 protein, mouseTransforming Growth Factor beta2

Identifiers

PMID35739142
PMCPMC9226129
OpenAlexW4283319984

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.