ArticlePLoS genetics2022
The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males.
Article in PLoS genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Rapid turnover of sex-determining architecture in invasive mussels.bioRxiv : the preprint server for biology · 2026Article
- Leveraging sex determination systems for genetic biocontrol of dipteran pests.Current opinion in insect science · 2026Review
- Mosquito sex separation using complementation of selectable traits and engineered neo-sex chromosomes.Nature communications · 2025Article
- Mosquito sex determination: recent advances and applications.Current opinion in insect science · 2025Review
- Insights on the sex determination, vector capacity and ecological biology from a chromosomal level genome of vector mosquito, Armigeres subulbatus.Infectious diseases of poverty · 2025Article
- Assessing AedesTech mosquito home system on yellow fever mosquito Aedes aegypti (Linnaeus) in Northern Malaysia.Journal of insect science (Online) · 2025Article
- On the Origin and Evolution of the Mosquito Male-determining Factor Nix.Molecular biology and evolution · 2024Article
- Use of Insect Promoters in Genetic Engineering to Control Mosquito-Borne Diseases.Biomolecules · 2022Review
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14 authors.
Funding
Abstract
Aedes albopictus is one of the most invasive insect species in the world and an effective vector for many important arboviruses. We reported previously that Ae. albopictus Nix (AalNix) is the male-determining factor of this species. However, whether AalNix alone is sufficient to initiate male development is unknown. Transgenic lines that express each of the three AalNix isoforms from the native promoter were obtained using piggyBac transformation. We verified the stable expression of AalNix isoforms in the transgenic lines and confirm that one isoform, AalNix3&4, is sufficient to convert females into fertile males (pseudo-males) that are indistinguishable from wild-type males. We also established a stable sex-converted female mosquito strain, AalNix3&4-♂4-pseudo-male. The pseudo-male mosquitoes can fly and mate normally with wild-type female, although their mating competitiveness is lower than wild-type. This work further clarifies the role of AalNix in the sex determination pathway and will facilitate the development of Ae. albopictus control strategies that rely on male-only releases such as SIT and sex-ratio distortion.
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