Evidence map›Paper›PMID 35737174›Full record

ArticleJournal of assisted reproduction and genetics2022

The effect of discrete wavelengths of visible light on the developing murine embryo.

Carl A Campugan, Megan Lim, Darren J X Chow, Tiffany C Y Tan, Tong Li, Avishkar A Saini, Antony Orth, Philipp Reineck, Erik P Schartner, Jeremy G Thompson and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
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  9. Investigation of refractive index dynamics duringBiomedical optics express · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 4 countries.

Carl A CampuganSchool of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.ORCID http://orcid.org/0000-0003-4947-8251
Megan LimSchool of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.ORCID http://orcid.org/0000-0002-9868-429X
Darren J X ChowSchool of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.ORCID http://orcid.org/0000-0002-2648-4600
Tiffany C Y TanSchool of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.ORCID http://orcid.org/0000-0002-8290-688X
Tong LiSchool of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.
Avishkar A SainiSchool of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.ORCID http://orcid.org/0000-0002-2221-7476
Antony OrthNational Research Council of Canada, Ottawa, Ontario, Canada.ORCID http://orcid.org/0000-0001-5937-6254
Philipp ReineckAustralian Research Council Centre of Excellence for Nanoscale BioPhotonics, School of Science, Royal Melbourne Institute of Technology, Melbourne, VIC, 3000, Australia.ORCID http://orcid.org/0000-0003-1549-937X
Erik P SchartnerAustralian Research Council Centre of Excellence for Nanoscale BioPhotonics, The University of Adelaide, Adelaide, SA, 5005, Australia.ORCID http://orcid.org/0000-0003-1669-4302
Jeremy G ThompsonSchool of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.ORCID http://orcid.org/0000-0003-4941-7731
Kishan DholakiaSchool of Physics and Astronomy, University of St Andrews, North Haugh, Scotland , KY16 9SS, UK.ORCID http://orcid.org/0000-0001-6534-9009
Kylie R DunningSchool of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia. kylie.dunning@adelaide.edu.au.ORCID http://orcid.org/0000-0002-0462-6479
Australian Research Council · AUNational Research Council Canada · CAThe University of Adelaide · AUUniversity of St Andrews · GB

Funding

Australian Research Council FL210100099Australian Research Council, Discovery Early Career Award DE200100279Centre of Excellence for Nanoscale BioPhotonics, Australian Research Council CE140100003Engineering and Physical Sciences Research Council EP/P030017/1Hospital Research Foundation C-MCF-58-2019
6 · The paper itself

Abstract

purposeA current focus of the IVF field is non-invasive imaging of the embryo to quantify developmental potential. Such approaches use varying wavelengths to gain maximum biological information. The impact of irradiating the developing embryo with discrete wavelengths of light is not fully understood. Here, we assess the impact of a range of wavelengths on the developing embryo.

methodsMurine preimplantation embryos were exposed daily to wavelengths within the blue, green, yellow, and red spectral bands and compared to an unexposed control group. Development to blastocyst, DNA damage, and cell number/allocation to blastocyst cell lineages were assessed. For the longer wavelengths (yellow and red), pregnancy/fetal outcomes and the abundance of intracellular lipid were investigated.

resultsSignificantly fewer embryos developed to the blastocyst stage when exposed to the yellow wavelength. Elevated DNA damage was observed within embryos exposed to blue, green, or red wavelengths. There was no effect on blastocyst cell number/lineage allocation for all wavelengths except red, where there was a significant decrease in total cell number. Pregnancy rate was significantly reduced when embryos were irradiated with the red wavelength. Weight at weaning was significantly higher when embryos were exposed to yellow or red wavelengths. Lipid abundance was significantly elevated following exposure to the yellow wavelength.

conclusionOur results demonstrate that the impact of light is wavelength-specific, with longer wavelengths also impacting the embryo. We also show that effects are energy-dependent. This data shows that damage is multifaceted and developmental rate alone may not fully reflect the impact of light exposure.

Indexed as

BlastocystEmbryonic DevelopmentAnimalsEmbryo, MammalianFemaleFertilization in VitroHumansLightLipidsMicePregnancyLipidsBlastocystMicroscopyPhotodamagePhototoxicityPreimplantation embryo

Identifiers

PMID35737174
PMCPMC9428105
OpenAlexW4283322796

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.