Evidence map›Paper›PMID 35737057›Full record

ArticleToxins2022

In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in

Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E Webb, Gerald Striedner, Winfried Römer, W Bruce Turnbull, Juergen Mairhofer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxins, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Natalia DanielewiczenGenes Biotech GmbH, Mooslackengasse 17, 1190 Vienna, Austria.
Wenyue DaiSchool of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
Francesca RosatoFaculty of Biology, University of Freiburg, Schänzlestraße 1, 79104 Freiburg, Germany.
Michael E WebbSchool of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
Gerald StriednerDepartment of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, 1190 Vienna, Austria.ORCID 0000-0001-8366-5195
Winfried RömerFaculty of Biology, University of Freiburg, Schänzlestraße 1, 79104 Freiburg, Germany.ORCID 0000-0002-2847-246X
W Bruce TurnbullSchool of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.ORCID 0000-0002-7352-0360
Juergen MairhoferenGenes Biotech GmbH, Mooslackengasse 17, 1190 Vienna, Austria.

Funding

Marie Curie 814029
6 · The paper itself

Abstract

Non-toxic derivatives of the cholera toxin are extensively used in neuroscience, as neuronal tracers to reveal the location of cells in the central nervous system. They are, also, being developed as vaccine components and drug-delivery vehicles. Production of cholera-toxin derivatives is often non-reproducible; the quality and quantity require extensive fine-tuning to produce them in lab-scale settings. In our studies, we seek a resolution to this problem, by expanding the molecular toolbox of the

Indexed as

Cholera ToxinEscherichia coliHumansCholera Toxinaffinity chromatographycholera toxinfed-batch processGM1 receptorGspDholotoxin-like chimaeraneuronal tracerretrograde transportT2SS machinery

Identifiers

PMID35737057
PMCPMC9228256

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.