ArticleMicroscopy research and technique2022
Measuring biological materials mechanics with atomic force microscopy - Determination of viscoelastic cell properties from stress relaxation experiments.
Article in Microscopy research and technique, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- A Hybrid Hierarchical Framework for Quantifying Mechanical Markers in Atherosclerotic Disease Progression: A New Approach for Diagnosis and Risk Assessment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Lectin-Micropatterned Biointerfaces Regulate Cytoskeletal Organization and Viscoelastic Phenotypes of Bladder Cancer Cells.ACS omega · 2026Article
- Scale-Specific Viscoelastic Characterization of Hydrogels: Integrated AFM and Finite Element Modeling.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Mapping single-cell rheology of ascidian embryos in the cleavage stages using AFM.Biophysical journal · 2025Article
- Viscoelastic characterization of the human osteosarcoma cancer cell line MG-63 using a fractional-order zener model through automated algorithm design and configuration.Scientific reports · 2025Article
- Effects of Shear and Extensional Stresses on Cells: Investigation in a Spiral Microchannel and Contraction-Expansion Arrays.ACS biomaterials science & engineering · 2025Review
- Review
- Theoretical and Experimental Studies of the Dynamic Damage of Endothelial Cellular Networks Under Ultrasound Cavitation.Cellular and molecular bioengineering · 2025Article
- The mechanopathology of the tumor microenvironment: detection techniques, molecular mechanisms and therapeutic opportunities.Frontiers in cell and developmental biology · 2025Review
- Atomic Force Microscopy for the Study of Cell Mechanics in Pharmaceutics.Pharmaceutics · 2024Review
- E-cadherin adhesion dynamics as revealed by an accelerated force ramp are dependent upon the presence of α-catenin.Biochemical and biophysical research communications · 2023Article
- Review
- Application of self-organizing maps to AFM-based viscoelastic characterization of breast cancer cell mechanics.Scientific reports · 2023Article
- Power Laws Describe Bacterial Viscoelasticity.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Measuring biological materials mechanics with atomic force microscopy - Determination of viscoelastic cell properties from stress relaxation experiments.Microscopy research and technique · 2022Article
- Viscoelasticity during development: What is it? and why should you care?Seminars in cell & developmental biologyReview
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3 authors.
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Abstract
Cells are complex, viscoelastic bodies. Their mechanical properties are defined by the arrangement of semiflexible cytoskeletal fibers, their crosslinking, and the active remodeling of the cytoskeletal network. Atomic force microscopy (AFM) is an often-used technique for the study of cell mechanics, enabling time- and frequency-dependent measurements with nanometer resolution. Cells exhibit time-dependent deformation when stress is applied. In this work, we have investigated the stress relaxation of HeLa cells when subjected to a constant strain. We have varied the applied force (1, 2, 4, and 8 nN) and pause time (1, 10, and 60 s) to check for common assumptions for the use of models of linear viscoelasticity. Then, we have applied three models (standard linear solid, five element Maxwell, power law rheology) to study their suitability to fit the datasets. We show that the five element Maxwell model captures the stress relaxation response the best while still retaining a low number of free variables. This work serves as an introduction and guide when performing stress relaxation experiments on soft matter using AFM. RESEARCH HIGHLIGHTS: Cells exhibit linear viscoelastic properties when subjected to stress relaxation measurements at the studied different forces and times. The stress relaxation is best described by a five element Maxwell model. All three used models capture a softening and fluidization of cells when disrupting actin filaments.
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