Expression, Purification, and Characterisation of South African Cassava Mosaic Virus Cell-to-Cell Movement Protein.

Nikita Nankoo, Ikechukwu Anthony Achilonu, Marie Emma Christine Rey

Abstract read

In one paragraph

Article in Current issues in molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Bioinformatic-based approach for mutagenesis of plant immune Tm-2Frontiers in plant science · 2022
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nikita NankooPlant Biotechnology Research Laboratory, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg 2050, South Africa.

Ikechukwu Anthony AchilonuPlant Biotechnology Research Laboratory, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg 2050, South Africa.

Marie Emma Christine ReyPlant Biotechnology Research Laboratory, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg 2050, South Africa.

Funding

South African National Research Foundation 104829Wellcome Trust 104790

6 · The paper itself

Abstract

South African cassava mosaic virus (SACMV) is a circular ssDNA bipartite begomovirus, whose genome comprises DNA-A (encodes six genes) and DNA-B (encodes BC1 cell-to-cell movement and BV1 nuclear shuttle proteins) components. A few secondary and tertiary structural and physicochemical characteristics of partial but not full-length begomovirus proteins have been elucidated to date. The full-length codon-optimised SACMV BC1 gene was cloned into a pET-28a (+) expression vector and transformed into expression host cells

Indexed as

ANScell-to-cell movement proteinextrinsic ANS fluorescenceintrinsic tryptophan fluorescencemant-ATPSouth African cassava mosaic virus (SACMV)

Identifiers

PMID35735627

DOI10.3390/cimb44060186

PMCPMC9221656

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.