Evidence map›Paper›PMID 35734849›Full record

ArticleAngewandte Chemie (International ed. in English)2022

A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.

Sergi Roda, Laura Fernandez-Lopez, Marius Benedens, Alexander Bollinger, Stephan Thies, Julia Schumacher, Cristina Coscolín, Masoud Kazemi, Gerard Santiago, Christoph G W Gertzen and 6 more

Open access · hybridAbstract read
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
3.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 32 citations in OpenAlex.

  1. Journal of chemical information and modeling · 2026
    Article
  2. EngineeringiScience · 2026
    Article
  3. Review
  4. Chemical Modification ofInternational journal of molecular sciences · 2025
    Article
  5. Chemoenzymatic synthesis.Communications chemistry · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Immobilization of Lipase B fromInternational journal of molecular sciences · 2022
    Article
  12. Article
  13. Design and Characterization of In-One Protease-Esterase PluriZyme.International journal of molecular sciences · 2022
    Article
  14. Article
  15. Article
  16. A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.Angewandte Chemie (International ed. in English) · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 2 countries.

Sergi RodaDepartment of Life Sciences, Barcelona Supercomputing Center, Carrer de Jordi Girona, 31, 08034, Barcelona, Spain.ORCID 0000-0002-0174-7435
Laura Fernandez-LopezDepartment of Applied Biocatalysis, ICP, CSIC, Marie Curie 2, 28049, Madrid, Spain.ORCID 0000-0001-8861-3191
Marius BenedensCenter for Structural Studies, Heinrich-Heine-University, Building 26.44.01.62, Universitaetsstr 1, 40228, Duesseldorf, Germany.
Alexander BollingerInstitute of Molecular Enzyme Technology, Heinrich-Heine-Universität Düsseldorf, Building 26.44.01.62, Universitaetsstr 1, 40228, Duesseldorf, Germany.ORCID 0000-0002-1349-7381
Stephan ThiesInstitute of Molecular Enzyme Technology, Heinrich-Heine-Universität Düsseldorf, Building 26.44.01.62, Universitaetsstr 1, 40228, Duesseldorf, Germany.ORCID 0000-0003-4240-9149
Julia SchumacherCenter for Structural Studies, Heinrich-Heine-University, Building 26.44.01.62, Universitaetsstr 1, 40228, Duesseldorf, Germany.
Cristina CoscolínDepartment of Applied Biocatalysis, ICP, CSIC, Marie Curie 2, 28049, Madrid, Spain.ORCID 0000-0002-2117-8911
Masoud KazemiDepartment of Life Sciences, Barcelona Supercomputing Center, Carrer de Jordi Girona, 31, 08034, Barcelona, Spain.ORCID 0000-0002-0750-8865
Gerard SantiagoDepartment of Life Sciences, Barcelona Supercomputing Center, Carrer de Jordi Girona, 31, 08034, Barcelona, Spain.ORCID 0000-0002-0506-3049
Christoph G W GertzenCenter for Structural Studies, Heinrich-Heine-University, Building 26.44.01.62, Universitaetsstr 1, 40228, Duesseldorf, Germany.ORCID 0000-0002-9562-7708
Jose L Gonzalez-AlfonsoDepartment of Applied Biocatalysis, ICP, CSIC, Marie Curie 2, 28049, Madrid, Spain.ORCID 0000-0002-3396-7985
Francisco J PlouDepartment of Applied Biocatalysis, ICP, CSIC, Marie Curie 2, 28049, Madrid, Spain.ORCID 0000-0003-0831-893X
Karl-Erich JaegerInstitute of Molecular Enzyme Technology, Heinrich-Heine-Universität Düsseldorf, Building 26.44.01.62, Universitaetsstr 1, 40228, Duesseldorf, Germany.ORCID 0000-0002-6036-0708
Sander H J SmitsCenter for Structural Studies, Heinrich-Heine-University, Building 26.44.01.62, Universitaetsstr 1, 40228, Duesseldorf, Germany.ORCID 0000-0003-0780-9251
Manuel FerrerDepartment of Applied Biocatalysis, ICP, CSIC, Marie Curie 2, 28049, Madrid, Spain.ORCID 0000-0003-4962-4714
Víctor GuallarDepartment of Life Sciences, Barcelona Supercomputing Center, Carrer de Jordi Girona, 31, 08034, Barcelona, Spain.ORCID 0000-0002-4580-1114
Instituto de Catálisis y Petroleoquímica · ESHeinrich Heine University Düsseldorf · DEBarcelona Supercomputing Center · ESForschungszentrum Jülich · DEInstitució Catalana de Recerca i Estudis Avançats · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Engineering dual-function single polypeptide catalysts with two abiotic or biotic catalytic entities (or combinations of both) supporting cascade reactions is becoming an important area of enzyme engineering and catalysis. Herein we present the development of a PluriZyme, TR

Indexed as

Amino AcidsTransaminasesCatalysisEsterasesEstersHydrolysisAmino AcidsEsterasesEstersTransaminasesCascade ReactionsComputational EngineeringEsterasePlurizymeTransaminase

Identifiers

PMID35734849
PMCPMC9540564
OpenAlexW4283392446

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.