ArticleFrontiers in plant science2022
Identification of Reference Genes for Reverse Transcription-Quantitative PCR Analysis of Ginger Under Abiotic Stress and for Postharvest Biology Studies.
Article in Frontiers in plant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 53 citations in OpenAlex.
- Contrasting roles of VfSBP transcription factors in regulating disease resistance and abiotic stress tolerance in faba bean.Plant cell reports · 2026Article
- A comparative analysis of root system characteristics, endogenous hormones, and transcriptome responses in tissue cultured ginger.BMC plant biology · 2026Article
- Evaluation and identification of reference genes for qRT-PCR analysis in bermudagrass roots under alkaline salt stress.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Genome-wide characterization and expression analysis of the bHLH gene family in response to abiotic stresses in Zingiber officinale Roscoe.BMC genomics · 2025Article
- Identification and validation of stable reference genes for RT-qPCR analyses of Kobresia littledalei seedlings.BMC plant biology · 2024Article
- Reference genes for Eucalyptus spp. under Beauveria bassiana inoculation and subsequently infestation by the galling wasp Leptocybe invasa.Scientific reports · 2024Article
- Genome-wide analysis and expression pattern of the ZoPP2C gene family in Zingiber officinale Roscoe.BMC genomics · 2024Article
- Genome-Wide Identification of DUF668 Gene Family and Expression Analysis underInternational journal of molecular sciences · 2024Article
- Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns ofInternational journal of molecular sciences · 2023Article
- Integrated analyses of metabolomics and transcriptomics reveal the potential regulatory roles of long non-coding RNAs in gingerol biosynthesis.BMC genomics · 2023Article
- Systematic identification of reference genes for qRT-PCR ofFrontiers in plant science · 2023Article
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Authors and funding
11 authors at 3 institutions in 1 country.
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Abstract
Gene expression analysis largely improves our understanding of the molecular basis underpinning various plant biological processes. Stable reference genes play a foundational role during the normalization of gene expression levels. However, until now, there have been few reference genes suitable for ginger reverse transcription-quantitative PCR (RT-qPCR) research. In this study, 29 candidate reference genes with stable expression patterns across multiple ginger tissues and 13 commonly used reference genes were selected to design RT-qPCR primers. After amplification specificity validation, 32 candidates were selected and further evaluated by RT-qPCR using samples from various organs subjected to NaCl, drought, heat, waterlogging, and chilling stress. Four strategies, including delta-CT, BestKeeper, geNorm, and NormFinder, were used to rank the stability of reference genes, and the ranks produced by these four strategies were comprehensively evaluated by RefFinder to determine the final rank. Overall, the top three stability reference genes indicated by RefFinder were
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