Evidence map›Paper›PMID 35733252›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2022

Metal cofactor stabilization by a partner protein is a widespread strategy employed for amidase activation.

Julia E Page, Meredith A Skiba, Truc Do, Andrew C Kruse, Suzanne Walker

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

  1. A conserved hub protein required for peptidoglycan remodeling and cell division inProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Julia E PageDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.ORCID 0000-0002-9884-2382
Meredith A SkibaDepartment of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.ORCID 0000-0003-4615-6775
Truc DoDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.ORCID 0000-0001-7212-0296
Andrew C KruseDepartment of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.ORCID 0000-0002-1467-1222
Suzanne WalkerDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.ORCID 0000-0002-0545-914X
Harvard University · US

Funding

Medical Scientist Training ProgramT32GM007753 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI WALENSKY, LOREN DAVID · 1985 to 2021
$50.0M
User Training & OutreachP30GM138396 · NIGMS · UCHICAGO ARGONNE, LLC · PI JANET L. SMITH · 2020 to 2026
$34.3M
Mechanism and Inhibition of Enzymes that Assemble Bacterial Cell WallR01GM076710 · NIGMS · HARVARD MEDICAL SCHOOL · PI WALKER, SUZANNE · 2007 to 2019
$5.9M
Discovery and characterization of new bacterial cell wall targets and inhibitors to treat resistant infectionsR01AI148752 · NIAID · HARVARD MEDICAL SCHOOL · PI Daniel Kahne, Suzanne Walker · 2020 to 2026
$5.5M
Release of Extracellular DNA during Biofilm Formation in Staphylococcus aureusR01AI139011 · NIAID · HARVARD UNIVERSITY · PI KAHNE, DANIEL, LOSICK, RICHARD MARC · 2018 to 2022
$3.2M
High-Speed High-Sensitivity Detector for X-ray Micro-Crystallography at GM/CA@APSS10OD012289 · OD · UNIVERSITY OF CHICAGO · PI FISCHETTI, ROBERT F. · 2014 to 2014
$2.0M
Understanding the Regulation and Biological Roles of Peptidoglycan Hydrolases in Staphylococcus aureusF30AI156972 · NIAID · HARVARD MEDICAL SCHOOL · PI PAGE, JULIA ELAINE · 2021 to 2023
$142k
NIAID NIH HHS F30 AI156972NIAID NIH HHS R01 AI139011NIAID NIH HHS R01 AI148752NIGMS NIH HHS P30 GM138396NIGMS NIH HHS R01 GM076710NIGMS NIH HHS T32 GM007753NIH HHS S10 OD012289
6 · The paper itself

Abstract

Construction and remodeling of the bacterial peptidoglycan (PG) cell wall must be carefully coordinated with cell growth and division. Central to cell wall construction are hydrolases that cleave bonds in peptidoglycan. These enzymes also represent potential new antibiotic targets. One such hydrolase, the amidase LytH in

Indexed as

Bacterial ProteinsMembrane ProteinsMetalsN-Acetylmuramoyl-L-alanine AmidaseCell WallEnzyme ActivationEnzyme StabilityPeptidoglycanProtein BindingProtein DomainsBacterial ProteinsLytH protein, Staphylococcus aureusMembrane ProteinsMetalsN-Acetylmuramoyl-L-alanine AmidasePeptidoglycanamidasecell wall hydrolasepeptidoglycantetratricopeptide repeat

Identifiers

PMID35733252
PMCPMC9245657
OpenAlexW4283326347

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.