Evidence map›Paper›PMID 35732937›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Studying Iridoid Transport in Catharanthus roseus by Grafting.

Maisha Farzana, Mohammadamin Shahsavarani, Vincenzo De Luca, Yang Qu

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Maisha FarzanaDepartment of Chemistry, University of New Brunswick, Fredericton, NB, Canada.
Mohammadamin ShahsavaraniDepartment of Chemical Engineering, University of New Brunswick, Fredericton, NB, Canada.
Vincenzo De LucaDepartment of Biological Sciences, Brock University, St. Catharines, ON, Canada.
Yang QuDepartment of Chemistry, University of New Brunswick, Fredericton, NB, Canada. yang.qu@unb.ca.
University of New Brunswick · CABrock University · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The plant Catharanthus roseus is well known for its spatial separation of iridoid and monoterpenoid indole alkaloid (MIA) biosynthesis at both intracellular and intercellular levels, collectively suggested by RNA in situ hybridization, enzymatic and transcriptomic studies using leaf epidermis, and fluorescent protein tagging studies. Although documented in other plant species, the long-distance transport of iridoid glycosides, such as secologanin, has not been known in C. roseus until a recent study suggested that secologanin is transported from root to shoot, by grafting low iridoid/MIA mutant scions onto wild-type stock plants. This chapter describes the in vitro cultivation of C. roseus plants and grafting techniques to enable studies concerning iridoid/MIA transport between organs. The iridoid and MIA analysis methods are also provided.

Indexed as

CatharanthusGene Expression Regulation, PlantIridoidsPlant LeavesPlant ProteinsTranscriptomeIridoidsPlant ProteinsCatharanthus roseusEMS mutagenesisGraftingSecologanin transport

Identifiers

PMID35732937
OpenAlexW4285245194

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.