ArticleScientific reports2022
Chicken-derived RSPO1 and WNT3 contribute to maintaining longevity of chicken intestinal organoid cultures.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- Chromatin accessibility of the jejunum in two high-yielding laying hen strains under divergent mineral phosphorus supply during the transition to egg laying.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Chicken-derived R-spondin1, Wnt3a, Nrg1 and human FGF1 allow branched growth of chicken intestinal organoid cultures.Poultry science · 2026Article
- Dynamic cellular heterogeneity revealed through a time-resolved single-cell atlas: assessment of porcine intestinal organoids as an in vitro model for deoxynivalenol and zearalenone.Journal of animal science and biotechnology · 2026Article
- Kinase inhibitors in organoid media influenceMicrobiology spectrum · 2026Article
- Organ-on-a-Chip: A Roadmap for Translational Research in Human and Veterinary Medicine.International journal of molecular sciences · 2025Review
- Novel organoids andParasitology · 2025Review
- Cultivating the endogenous life cycle ofParasitology · 2025Article
- Long-term culture of chicken tracheal organoids for the purpose of avian influenza virus research.Virology journal · 2025Article
- Cellular landscape of avian intestinal organoids revealed by single cell transcriptomics.Scientific reports · 2025Article
- An Adaptable Protocol to Generate a Murine Enteroid-Macrophage Co-Culture System.International journal of molecular sciences · 2024Article
- Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function.Journal of animal science and biotechnology · 2024Article
- Article
- Chicken intestinal organoids: a novel method to measure the mode of action of feed additives.Frontiers in immunology · 2024Article
- Differential Salmonella Typhimurium intracellular replication and host cell responses in caecal and ileal organoids derived from chicken.Veterinary research · 2023Article
- Temporal transcriptome profiling of floating apical out chicken enteroids suggest stability and reproducibility.Veterinary research · 2023Article
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intestinal organoids are advanced cellular models, which are widely used in mammalian studies to mimic and study in vivo intestinal function and host-pathogen interactions. Growth factors WNT3 and RSPO1 are crucial for the growth of intestinal organoids. Chicken intestinal organoids are currently cultured with mammalian Wnt3a and Rspo1, however, maintaining their longevity has shown to be challenging. Based on the limited homology between mammalian and avian RSPO1, we expect that chicken-derived factors are required for the organoid cultures. Isolated crypts from embryonic tissue of laying hens were growing in the presence of chicken WNT3 and RSPO1, whereas growth in the presence of mammalian Wnt3a and Rspo1 was limited. Moreover, the growth was increased by using Prostaglandin E2 (PGE
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