Evidence map›Paper›PMID 35732745›Full record

ArticleAnalytical and bioanalytical chemistry2022

Long-term continuous monitoring of microRNA in living cells using modified gold nanoprobe.

Yuling Liang, Huihui Yang, Wen Yin, Yanfei Zhang, Yuzhi Xu, Si-Yang Liu, Zong Dai, Xiaoyong Zou

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yuling LiangSchool of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Huihui YangSchool of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Wen YinSchool of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Yanfei ZhangSchool of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Yuzhi XuScientific Research Center, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen, 518107, China.
Si-Yang LiuGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen, 518107, China. liusiyang@mail.sysu.edu.cn.
Zong DaiGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen, 518107, China. daizong@mail.sysu.edu.cn.
Xiaoyong ZouSchool of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.

Funding

National Natural Science Foundation of China 21801259National Natural Science Foundation of China 21974153Natural Science Foundation of Guangdong Province 2022A1515011318the Guangdong Science and Technology Plan Project Grant 2020B1212060077the Scientific Technology Project of Guangzhou City 202103000003the Scientific Technology Project of Shenzhen City JCYJ20200109142410170the Scientific Technology Project of Shenzhen City JCYJ20210324120601004
6 · The paper itself

Abstract

Long-term and continuous monitoring of the microRNA (miRNA) expression in living cells is essential in biomedical research, but it is currently limited by fast consumption and easy digestion of probes in the intracellular environment. Herein, we report polydopamine-modified gold nanoparticles (AuNPs@PDA) as protective and efficient nanocarriers for DNA hairpin probes (hpDNA), achieving long-term monitoring (48 h) of the miRNA (let-7a) levels in living cells after drug treatments. This method enabled excellent sensitivity and high selectivity toward let-7a with a limit of detection of 0.51 nM (n = 3) and a linear range from 1 to 100 nM. More importantly, AuNPs@PDA can not only efficiently improve the loading of hpDNA on each nanoparticle, but also effectively protect hpDNA from hydrolysis in the cell microenvironment, finally realizing the continuous monitoring of let-7a in living cells for 48 h. This simple method would be of great significance for drug screening and precision medicine.

Indexed as

Metal NanoparticlesMicroRNAsDNA ProbesGoldLimit of DetectionDNA ProbesGoldMicroRNAsDNA hairpinFluorescence imagingGold nanoparticlesLong-term monitoringMicroRNA

Identifiers

PMID35732745

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.