ArticleScientific reports2022
Evaluation of within-host evolution of methicillin-resistant Staphylococcus aureus (MRSA) by comparing cgMLST and SNP analysis approaches.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 59 citations in OpenAlex.
- Genetic diversity and within-farm transmission of Staphylococcus aureus from ewes in Norway.BMC microbiology · 2026Article
- Population genomic variation inMicrobial genomics · 2026Article
- CharacterizingmSystems · 2025Article
- Review
- Comparison of core genome multi-locus sequencing typing pipelines for hospital outbreak detection of common bacterial pathogens.Journal of clinical microbiology · 2025Article
- A multi-hospital, clinician-initiated bacterial genomics programme to investigate treatment failure in severe Staphylococcus aureus infections.Nature communications · 2025Article
- Evaluation of nationwide analysis surveillance for methicillin-resistantMicrobial genomics · 2025Article
- Genomic analysis of community-associated methicillin-resistantFrontiers in microbiology · 2025Article
- A rare waterborne outbreak ofEmerging microbes & infections · 2024Article
- Within-host genomic evolution of methicillin-resistant Staphylococcus aureus in long-term carriers.Applied microbiology and biotechnology · 2024Article
- Unraveling the impact of genome assembly on bacterial typing: a one health perspective.BMC genomics · 2024Article
- The genetic relationship between human and pet isolates: a core genome multilocus sequence analysis of multidrug-resistant bacteria.Antimicrobial resistance and infection control · 2024Article
- SaLTy: a novelMicrobial genomics · 2024Article
- The intra-host evolutionary landscape and pathoadaptation of persistentMicrobial genomics · 2023Article
- Genomic Evidence for Direct Transmission ofAntibiotics (Basel, Switzerland) · 2023Article
- High Prevalence of Livestock-Associated Methicillin-ResistantTransboundary and emerging diseases · 2023Article
- Low-level colonization of methicillin-resistant Staphylococcus aureus in pigs is maintained by slowly evolving, closely related strains in Finnish pig farms.Acta veterinaria Scandinavica · 2022Article
- Study of SarA by DNA Affinity Capture Assay (DACA) Employing Three Promoters of Key Virulence and Resistance Genes in Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole genome sequencing (WGS) of methicillin-resistant Staphylococcus aureus (MRSA) provides high-resolution typing, facilitating surveillance and outbreak investigations. The aim of this study was to evaluate the genomic variation rate in MRSA, by comparing commonly used core genome multilocus sequencing (cgMLST) against single nucleotide polymorphism (SNP) analyses. WGS was performed on 95 MRSA isolates, collected from 20 carriers during years 2003-2019. To assess variation and methodological-related differences, two different cgMLST schemes were obtained using Ridom SeqSphere+ and the cloud-based 1928 platform. In addition, two SNP methods, 1928 platform and Northern Arizona SNP Pipeline (NASP) were used. The cgMLST using Ridom SeqSphere+ and 1928 showed a median of 5.0 and 2.0 allele variants/year, respectively. In the SNP analysis, performed with two reference genomes COL and Newman, 1928 showed a median of 13 and 24 SNPs (including presumed recombination) and 3.8 respectively 4.0 SNPs (without recombination) per individual/year. Accordantly, NASP showed a median of 5.5 and 5.8 SNPs per individual/year. In conclusion, an estimated genomic variation rate of 2.0-5.8 genetic events per year (without recombination), is suggested as a general guideline to be used at clinical laboratories for surveillance and outbreak investigations independently of analysis approach used.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.