Evidence map›Paper›PMID 35732323›Full record

ArticleCancer genomics & proteomics

Requirement of CLIC4 Expression in Human Colorectal Cancer Cells for Sensitivity to Growth Inhibition by Fucoxanthinol.

Reo Yokoyama, Ayumi Kushibiki, Shiori Yamada, Atsuhito Kubota, Hiroyuki Kojima, Tohru Ohta, Junichi Hamada, Hayato Maeda, Michihiro Mutoh, Masaru Terasaki

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer genomics & proteomics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Reo YokoyamaSchool of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan.
Ayumi KushibikiSchool of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan.
Shiori YamadaSchool of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan.
Atsuhito KubotaSchool of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan.
Hiroyuki KojimaSchool of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan.
Tohru OhtaAdvanced Research Promotion Center, Health Sciences University of Hokkaido, Hokkaido, Japan.
Junichi HamadaAdvanced Research Promotion Center, Health Sciences University of Hokkaido, Hokkaido, Japan.
Hayato MaedaFaculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan.
Michihiro MutohDepartment of Molecular-Targeting Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Masaru TerasakiSchool of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan; terasaki@hoku-iryo-u.ac.jp.
Health Sciences University of Hokkaido · JPHirosaki University · JPKyoto Prefectural University of Medicine · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimFucoxanthinol (FxOH), a marine carotenoid, induces apoptosis and anoikis in human colorectal cancer (CRC) DLD-1 cells via the down-regulation of chloride intracellular channel 4 (CLIC4) expression, a key molecule for apoptosis. However, whether FxOH is susceptible to CLIC4 expression and its regulatory mechanisms in human CRC cells remains unknown. We investigated the inhibitory effects of FxOH on six types of human CRC cells with CLIC4 regulation. MATERIALS AND

methodsThe association between FxOH and CLIC4 was investigated using gene knockdown, overexpression, and transcriptome analyses.

resultsCLIC4 expression in CRC cells was a significant factor associated with sensitivity to FxOH. CLIC4 regulates many cancer-related signals and participates in growth inhibition in FxOH-treated DLD-1 cells. Both CLIC4 knockdown and overexpression attenuated the inhibitory effects of FxOH on DLD-1 cells.

conclusionOur findings suggest that the protein expression of CLIC4 and its regulating mechanisms play significant roles regarding cell death in human CRC cells by FxOH treatment. Further investigation by in vitro and in vivo models is needed to determine the effect of CLIC4.

Indexed as

beta CaroteneChloride ChannelsColorectal NeoplasmsAnoikisCell Line, TumorHumansbeta CaroteneChloride ChannelsCLIC4 protein, humanfucoxanthinolCarotenoidchloride intracellular channel 4colorectal cancer cellsfucoxanthinfucoxanthinol

Identifiers

PMID35732323
PMCPMC9247876
OpenAlexW4283370637

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.