ArticleAnalytical chemistry2022
Single-Cell Chemical Proteomics (SCCP) Interrogates the Timing and Heterogeneity of Cancer Cell Commitment to Death.
Article in Analytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Optimized Sample Handling Minimizes Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics.Proteomics · 2026Article
- What Are the Practical Applications of Single-Cell Proteomics?Proteomes · 2026Article
- Editorial: Multiomics advances: bridging gaps in understanding intricate biological systems.Frontiers in plant science · 2026Article
- The applications of single-cell multiomics in drug screening.Pharmaceutical science advances · 2025Review
- Trends in Mass Spectrometry-Based Single-Cell Proteomics.Analytical chemistry · 2025Review
- Multifaceted Proteome Analysis at Solubility, Redox, and Expression Dimensions for Target Identification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Proteome-scale tissue mapping using mass spectrometry based on label-free and multiplexed workflows.bioRxiv : the preprint server for biology · 2024Article
- AM-DMF-SCP: Integrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip.JACS Au · 2024Article
- Efficient and Sensitive Sample Preparation, Separations, and Data Acquisition for Label-Free Single-Cell Proteomics.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A Multiplexed Single-Cell Proteomic Workflow Applicable to Drug Treatment Studies.Methods in molecular biology (Clifton, N.J.) · 2024Article
- EquiCP: Targeted Single-Cell Proteomics by Mass Spectrometry with Isobaric Labeled Multiplexing.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Analyzing Posttranslational Modifications in Single Cells.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Robust Surfactant-Assisted One-Pot Sample Preparation for Label-Free Single-Cell and Nanoscale Proteomics.Methods in molecular biology (Clifton, N.J.) · 2024Article
- An integrated method for single cell proteomics with simultaneous measurements of intracellular drug concentration implicates new mechanisms for adaptation to KRASbioRxiv : the preprint server for biology · 2023Article
- A review of the current state of single-cell proteomics and future perspective.Analytical and bioanalytical chemistry · 2023Review
- Toward Single Bacterium Proteomics.Journal of the American Society for Mass Spectrometry · 2023Article
- Recent advancements in single-cell metabolic analysis for pharmacological research.Journal of pharmaceutical analysis · 2023Review
- Systems-level temporal immune-metabolic profile in Crimean-Congo hemorrhagic fever virus infection.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Proteomics-Driven Biomarkers in Pancreatic Cancer.Proteomes · 2023Review
- Label-free single cell proteomics utilizing ultrafast LC and MS instrumentation: A valuable complementary technique to multiplexing.Proteomics · 2023Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemical proteomics studies the effects of drugs upon a cellular proteome. Due to the complexity and diversity of tumors, the response of cancer cells to drugs is also heterogeneous, and thus, proteome analysis at the single-cell level is needed. Here, we demonstrate that single-cell proteomics techniques have become quantitative enough to tackle the drug effects on target proteins, enabling single-cell chemical proteomics (SCCP). Using SCCP, we studied here the time-resolved response of individual adenocarcinoma A549 cells to anticancer drugs methotrexate, camptothecin, and tomudex, revealing the early emergence of cellular subpopulations committed and uncommitted to death. As a novel and useful approach to exploring the heterogeneous response to drugs of cancer cells, SCCP may prove to be a breakthrough application for single-cell proteomics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.