ArticleThe Journal of cell biology2022
A two-step search and run response to gradients shapes leukocyte navigation in vivo.
Article in The Journal of cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Cell-autonomous control coupled with tissue context regulates the cessation of migration at the site of organ development.Development (Cambridge, England) · 2026Article
- Chemical and Mechanical Regulation of Leukocyte Migration.Cold Spring Harbor perspectives in biology · 2026Review
- The Arp2/3 complex controls the development of homeostatic microglia.EMBO reports · 2026Article
- Shaping neutrophil morphology and function: the importance of a segmented nucleus.Nature reviews. Immunology · 2026Review
- cPLAScience advances · 2026Article
- Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation.PLoS computational biology · 2026Article
- Trade-off between branching and polarity controls decision-making during cell migration.Science advances · 2026Article
- Characterizing trajectories of innate immune cells in larval zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Macrophages migrate persistently and directionally upon entering 2D confinement in the presence of extracellular matrix.Biology open · 2025Article
- Macrophages migrate persistently and directionally upon entering 2D confinement in the presence of extracellular matrix.bioRxiv : the preprint server for biology · 2025Article
- Live imaging in zebrafish reveals tissue-specific strategies for amoeboid migration.Development (Cambridge, England) · 2025Article
- Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration.Nature communications · 2025Article
- Visualising Neutrophil Actin Dynamics in Zebrafish in Response to Laser Wounding Using Two-Photon Microscopy.Bio-protocol · 2024Article
- Arp2/3 complex and the pentose phosphate pathway regulate late phases of neutrophil swarming.iScience · 2024Article
- Migrate3D: Software for simplified post-tracking analysis of 3D and 2D cell migration data.Research square · 2024Article
- Neutrophils under the microscope: neutrophil dynamics in infection, inflammation, and cancer revealed using intravital imaging.Frontiers in immunology · 2024Review
- Exploring the dynamic behavior of leukocytes with zebrafish.Current opinion in cell biology · 2023Review
- Local negative feedback of Rac activity at the leading edge underlies a pilot pseudopod-like program for amoeboid cell guidance.PLoS biology · 2023Article
- The circadian neutrophil, inside-out.Journal of leukocyte biology · 2023Review
- The cell's dilemma: resolving directional decisions.Trends in immunology · 2023Article
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5 authors.
Funding
Abstract
Migrating cells must interpret chemical gradients to guide themselves within tissues. A long-held principle is that gradients guide cells via reorientation of leading-edge protrusions. However, recent evidence indicates that protrusions can be dispensable for locomotion in some contexts, raising questions about how cells interpret endogenous gradients in vivo and whether other mechanisms are involved. Using laser wound assays in zebrafish to elicit acute endogenous gradients and quantitative analyses, we demonstrate a two-stage process for leukocyte chemotaxis in vivo: first a "search" phase, with stimulation of actin networks at the leading edge, cell deceleration, and turning. This is followed by a "run" phase, with fast actin flows, cell acceleration, and persistence. When actin dynamics are perturbed, cells fail to resolve the gradient, suggesting that pure spatial sensing of the gradient is insufficient for navigation. Our data suggest that cell contractility and actin flows provide memory for temporal sensing, while expansion of the leading edge serves to enhance gradient sampling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.