ArticleMolecular ecology resources2022
Environmental nucleic acids: A field-based comparison for monitoring freshwater habitats using eDNA and eRNA.
Article in Molecular ecology resources, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Establishing Practical and Cost-Effective Methodologies for Preservation and Extraction of Environmental RNA (eRNA) From Aquatic Samples.Molecular ecology resources · 2026Article
- Biomonitoring 3.0: Beyond Taxa Lists to Evidence-Tiered Monitoring of Ecosystem Dynamics and Interactions.Ecology and evolution · 2026Review
- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- Automated eDNA and eRNA profiling for biodiversity monitoring in marine and freshwater ecosystems.Scientific reports · 2026Article
- Environmental RNA reflects seasonal changes in the metabolic activity of stream salamanders.Scientific reports · 2026Article
- Residual eDNA in eRNA Extracts Skews eRNA-Based Biodiversity Assessment: Call for Optimised DNase Treatment.Molecular ecology resources · 2026Article
- Environmental DNA/RNA for non-invasive early detection and monitoring of pathogen dynamics in Atlantic salmon (Aquaculture (Amsterdam, Netherlands) · 2026Article
- Pattern of Detections Across Multiple Environmental Messenger RNAs (e-mRNAs) in Stressor-Exposed Zebrafish (Ecology and evolution · 2026Article
- Article
- Environmental RNA-Based Metatranscriptomics as a Novel Biomonitoring Tool: A Case Study of Glyphosate-Based Herbicide Effects on Freshwater Eukaryotic Communities.Molecular ecology · 2025Article
- VoronaGasyCodes: A Public Database of Mitochondrial Barcodes for Malagasy Birds.Molecular ecology resources · 2025Article
- Comparative Decay Dynamics and Detectability of eDNA and eRNA in Connected and Isolated Freshwater Mesocosms Using Digital PCR.Molecular ecology resources · 2025Article
- Differential decay of multiple environmental nucleic acid components.Scientific reports · 2025Article
- Environmental transcriptomics under heat stress: Can environmental RNA reveal changes in gene expression of aquatic organisms?Molecular ecology · 2025Article
- Quencher-free CRISPR-based molecular detection using an amphiphilic DNA fluorescence probe.Biosensors & bioelectronics · 2025Article
- Evaluating eDNA and eRNA metabarcoding for aquatic biodiversity assessment: From bacteria to vertebrates.Environmental science and ecotechnology · 2024Article
- Larger particle size distribution of environmental RNA compared to environmental DNA: a case study targeting the mitochondrial cytochrome b gene in zebrafish (Danio rerio) using experimental aquariums.Die Naturwissenschaften · 2024Article
- New prospects of environmental RNA metabarcoding research in biological diversity, ecotoxicological monitoring, and detection of COVID-19: a critical review.Environmental science and pollution research international · 2024Review
- Article
- Methodological considerations for aqueous environmental RNA collection, preservation, and extraction.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
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Authors and funding
3 authors.
Funding
Abstract
Nucleic acids released by organisms and isolated from environmental substrates are increasingly being used for molecular biomonitoring. While environmental DNA (eDNA) has received much attention, the potential of environmental RNA as a biomonitoring tool remains under-explored. Several recent studies using paired DNA and RNA metabarcoding of bulk samples suggest that RNA might better reflect "metabolically active" parts of the community. However, such studies mainly capture organismal eDNA and eRNA. For larger eukaryotes, isolation of extra-organismal RNA will be important, but viability needs to be examined in a field-based setting. In this study we evaluate (a) whether extra-organismal eRNA release from macroeukaryotes can be detected given its supposedly rapid degradation, and (b) if the same field collection methods for eDNA can be applied to eRNA. We collected eDNA and eRNA from water in lakes where fish community composition is well documented, enabling a comparison between the two nucleic acids in two different seasons with monitoring using conventional methods. We found that eRNA is released from macroeukaryotes and can be filtered from water and metabarcoded in a similar manner as eDNA to reliably provide species composition information. eRNA had a small but significantly greater true positive rate than eDNA, indicating that it correctly detects more species known to exist in the lakes. Given relatively small differences between the two molecules in describing fish community composition, we conclude that if eRNA provides significant advantages in terms of lability, it is a strong candidate to add to the suite of molecular monitoring tools.
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