Evidence map›Paper›PMID 35729244›Full record

ArticleScientific reports2022

RNAi-induced knockdown of white gene in the southern green stink bug (Nezara viridula L.).

Dariane Souza, Shawn A Christensen, Ke Wu, Lyle Buss, Kaylin Kleckner, Constance Darrisaw, Paul D Shirk, Blair D Siegfried

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
4.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Article
  3. Characterization of Ionotropic Receptor GenePlants (Basel, Switzerland) · 2023
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Dariane SouzaEntomology and Nematology Department, University of Florida, Gainesville, 32611, USA. dariane.souza@syngenta.com.
Shawn A ChristensenUSDA-ARS Center for Medical, Agricultural and Veterinary Entomology, Gainesville, 32608, USA.
Ke WuEntomology and Nematology Department, University of Florida, Gainesville, 32611, USA.
Lyle BussEntomology and Nematology Department, University of Florida, Gainesville, 32611, USA.
Kaylin KlecknerEntomology and Nematology Department, University of Florida, Gainesville, 32611, USA.
Constance DarrisawEntomology and Nematology Department, University of Florida, Gainesville, 32611, USA.
Paul D ShirkUSDA-ARS Center for Medical, Agricultural and Veterinary Entomology, Gainesville, 32608, USA.
Blair D SiegfriedEntomology and Nematology Department, University of Florida, Gainesville, 32611, USA.
University of Florida · USCenter for Medical, Agricultural and Veterinary Entomology · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The southern green stink bug (SGSB) Nezara viridula L. is one of the most common stink bug species in the United States and can cause significant yield loss in a variety of crops. A suitable marker for the assessment of gene-editing tools in SGSB has yet to be characterized. The white gene, first documented in Drosophila, has been a useful target to assess the efficiency of introduced mutations in many species as it controls pigmentation processes and mutants display readily identifiable phenotypes. In this study we used the RNAi technique to investigate functions and phenotypes associated with the white ortholog in the SGSB and to validate white as a marker for genetic transformation in this species. This study revealed that white may be a suitable marker for germline transformation in the SGSB as white transcript knockdown was not lethal, did not impair embryo development and provided a distinguishable phenotype. Our results demonstrated that the white ortholog in SGSB is involved in the pathway for ommochrome synthesis and suggested additional functions of this gene such as in the integument composition, management of hemolymph compounds and riboflavin mobilization.

Indexed as

HeteropteraAnimalsCrops, AgriculturalRNA Interference

Identifiers

PMID35729244
PMCPMC9213411
OpenAlexW4283210350

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.