ArticleRapid communications in mass spectrometry : RCM2022
Rapid and flexible online desalting using Nafion-coated melamine sponge for mass spectrometry analysis.
Article in Rapid communications in mass spectrometry : RCM, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Improved Detection of Polysulfated Oligosaccharides by Mass Spectrometry Applicable to Miniaturized Samples.Molecules (Basel, Switzerland) · 2024Article
- Unlocking the potential of 3D printed microfluidics for mass spectrometry analysis using liquid infused surfaces.Analytica chimica acta · 2023Article
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Authors and funding
5 authors.
Funding
Abstract
rationaleThe performance of mass spectrometry (MS) analysis is often affected by the presence of salt ions. To achieve optimal MS detection results, desalting is necessary for samples with high salt concentrations. We report a rapid, low-cost and flexible online desalting method using Nafion-coated sponge. This method is easy to perform and can be implemented to a wide range of customized fluidic systems.
methodsNafion-coated melamine sponge was fabricated by soaking a glass tube containing a melamine sponge in Nafion solution and then drying overnight. The online desalting workflow is comprised of three major parts: (1) Syringe pump, which provides a continuous flow for the online fluid system; (2) Nafion sponge in a glass tube, where the online desalting of sample solution happens; (3) Capillary Vibrating Sharp-Edge Spray Ionization (cVSSI), which is an ionization technique to ionize the desalted analytes.
resultsEffective online desalting of a 10 mM NaCl solution was demonstrated for a wide range of molecules including small molecules, peptides, DNAs, and proteins using a flow rate of 10 μL/min. By incorporating multiple pieces of the Nafion-coated sponge, effective desalting for ubiquitin and cytochrome c (Cyt-c) from physiological buffers, including phosphate-buffered saline (PBS) and tris-buffered saline (TBS), were also achieved. For molecules that are sensitive to low pH conditions after desalting, a R-SO
conclusionsFlexible, low-cost, and efficient online desalting was achieved by the Nafion-coated sponge. A variety of molecules ranging from small molecules, peptides, proteins to oligosaccharides and DNAs can be desalted for MS analysis. The desalting by Nafion sponge has great potential for desalting applications that require customized fluidic design and rapid analysis.
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