Evidence map›Paper›PMID 35727561›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

In Vitro Assays for the Bifunctional Acylpeptide Hydrolase (APEH) Enzyme from Antarctic Fish.

Carmela Fusco, Bruna Agrillo, Marta Gogliettino, Gianna Palmieri, Ennio Cocca

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 82% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Carmela FuscoInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), Naples, Italy.
Bruna AgrilloInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), Naples, Italy.
Marta GogliettinoInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), Naples, Italy.
Gianna PalmieriInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), Naples, Italy.
Ennio CoccaInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), Naples, Italy. ennio.cocca@ibbr.cnr.it.
National Research Council · ITInstitute of Biosciences and Bioresources · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The bifunctional enzyme acylpeptide hydrolase (APEH) is involved in important metabolic processes both as an exopeptidase and as an endopeptidase. Hence, the growing interest in the study of this protein and the need to set up in vitro assays for its characterization. This chapter describes two in vitro assays able to detect the activities of APEH, one for the exopeptidase activity and one for the endopeptidase activity. In particular, these assays have been set up on the two APEH isoforms from Antarctic fish, characterized by a distinct functionality and marked exo- and endopeptidase activities.

Indexed as

FishesPeptide HydrolasesAnimalsAntarctic RegionsEndopeptidasesExopeptidasesProteolysisacylaminoacyl-peptidaseEndopeptidasesExopeptidasesPeptide HydrolasesAcylpeptide hydrolase (APEH)Antarctic fishIn vitro assayOxidized protein hydrolase (OPH)

Identifiers

PMID35727561
OpenAlexW4285251216

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.