Physical Mapping of Repeated Sequences on Fish Chromosomes by Fluorescence In Situ Hybridization (FISH).

Laura Ghigliotti, Juliette Auvinet, Eva Pisano

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Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

3 authors.

Laura GhigliottiNational Research Council of Italy (CNR), Institute for the Study of the Anthropic Impacts and the Sustainability of the Marine Environment (IAS), Genoa, Italy. laura.ghigliotti@cnr.it.

Juliette AuvinetDepartment of Marine and Environmental Sciences, Northeastern University, Marine Science Center, Nahant, MA, USA.

Eva PisanoNational Research Council of Italy (CNR), Institute for the Study of the Anthropic Impacts and the Sustainability of the Marine Environment (IAS), Genoa, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The opportunity to map genes and noncoding DNA sequences on the chromosomes by fluorescence in situ hybridization (FISH) has greatly enhanced the potential for fish karyotyping and comparative cytogenetics. The use of FISH allowed for significant advances in our understanding of the fish genome architecture, especially when applied to the study of the repetitive component of the genome, that is generally underestimated in the bioinformatic assembly. Here we provide a step-by-step protocol for FISH of repeated sequences onto chromosomes of fish species.

Indexed as

ChromosomesRepetitive Sequences, Nucleic AcidChromosome MappingIn Situ Hybridization, FluorescenceKaryotypingFishFluorescence microscopyGenome architectureRepetitive sequenceRibosomal geneTransposable element

Identifiers

PMID35727557

DOI10.1016/j.margen.2019.100737

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.