Evidence map›Paper›PMID 35727447›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Rapid Cloning of Random Mutagenesis Libraries Using PTO-QuickStep.

Pawel Jajesniak, Kang Lan Tee, Tuck Seng Wong

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Pawel JajesniakDepartment of Chemical and Biological Engineering, ChELSI Institute and Advanced Biomanufacturing Centre, University of Sheffield, Sheffield, England, UK.
Kang Lan TeeDepartment of Chemical and Biological Engineering, ChELSI Institute and Advanced Biomanufacturing Centre, University of Sheffield, Sheffield, England, UK.
Tuck Seng WongDepartment of Chemical and Biological Engineering, ChELSI Institute and Advanced Biomanufacturing Centre, University of Sheffield, Sheffield, England, UK. t.wong@sheffield.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/R020183/1
6 · The paper itself

Abstract

PTO-QuickStep is a quick and easy molecular cloning technique that allows seamless point integration of a DNA fragment, encoding either a tag or a protein, into any position within a target plasmid. The entire process is conducted in a time-efficient and cost-effective manner, without the need of DNA gel purification and enzymatic restriction and ligation. PTO-QuickStep further innovates protein engineering by providing the possibility of integrating a random mutagenesis step (e.g., error-prone PCR) into the workflow, without compromising the time duration required. Random mutagenesis libraries can be quickly and efficiently cloned into a plasmid of interest, thereby accelerating directed evolution. On top of that, PTO-QuickStep can be utilized for rapid integration of noncoding DNA fragments to modify existing plasmids, making it an excellent tool for synthetic biologists.

Indexed as

Cloning, MolecularDNAGene LibraryMutagenesisPlasmidsPolymerase Chain ReactionDNADirected evolutionDNA assemblyMetabolic engineeringMolecular cloningProtein engineeringRandom mutagenesisRecombinant DNASynthetic biology

Identifiers

PMID35727447

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.